| Field | Specification |
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| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human HIF-1-alpha was used as the immunogen for the HIF1A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HIF1A Antibody / HIF-1 alpha is a anti-HIF1A Rabbit antibody Recombinant Rabbit Monoclonal clone 31H88 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: HIF1A
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31H88, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF
Biological background
HIF1A antibody is widely applied in cancer research, cardiovascular biology, and metabolism. In tumors, HIF-1 alpha promotes angiogenesis by inducing VEGF and other pro angiogenic factors, supporting tumor growth and metastasis. In ischemic heart disease and stroke, HIF-1 alpha regulates protective adaptations. In metabolism, HIF-1 alpha shifts energy production toward glycolysis, contributing to the Warburg effect in cancer. By detecting HIF-1 alpha, researchers can investigate how hypoxia drives physiological and pathological processes.
Western blot assays detect HIF-1 alpha stabilization in hypoxic cells. Immunohistochemistry maps nuclear accumulation in hypoxic tumor regions, while immunofluorescence highlights dynamic nuclear translocation. ELISA quantifies HIF-1 alpha levels in experimental and clinical samples. These assays make HIF1A antibody an essential reagent for hypoxia biology.
HIF-1 alpha controls genes involved in angiogenesis, erythropoiesis, glucose metabolism, and apoptosis. Dysregulation contributes to cancer progression, ischemic disease, and inflammatory disorders. Therapeutic strategies aim to inhibit HIF-1 alpha in cancer or enhance its activity in ischemic injury. By applying HIF1A antibody, scientists can monitor pathway activity and evaluate therapeutic interventions.
HIF1A antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
