HIF3A Antibody, FITC conjugated

Overview

This polyclonal anti-HIF3A antibody raised in Rabbit, conjugated with FITC. Designed to detect HIF3A in Human for cardiovascular research.

Key elements and design rationale

• Immunogen: Recombinant Human Hypoxia-inducible factor 3-alpha protein (516-669AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
• FITC: Direct fluorescence (Ex490/Em525); protect from light; use antifade.

Biological background

HIF3A (also: Basic-helix-loop-helix-PAS protein MOP7, bHLHe17, Class E basic helix-loop-helix protein 17, HIF 3A, HIF 3A4, HIF-3-alpha, HIF3 alpha, HIF3-alpha, HIF3-alpha-1, HIF3A, HIF3A_HUMAN, Hypoxia Inducible Factor 3 alpha, Hypoxia inducible factor 3 alpha subunit, Hypoxia inducible factor three alpha, Hypoxia-inducible factor 3-alpha, Inhibitory PAS domain protein, IPAS, Member of PAS protein 7, MOP7, PAS domain-containing protein 7, PASD7) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current HIF3A annotation relevant to cardiovascular.

Common research applications

• Refer to product documentation for confirmed application data.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12554900)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.