| Field | Specification |
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Hifair™ AdvanceFast 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA Digester Plus) is an upgraded version of the Hifair™ III SuperMix with enhanced inhibitor tolerance, faster reverse transcription speed, and higher cDNA yield. The upgraded gDNA Digester Mix-V2 effectively digests up to 1,000 ng residual genomic DNA. Total workflow time is approximately 7 minutes (5-min RT step). Suitable for RNA from animals, plants, and microorganisms.
Features
- Ultrafast reverse transcription: complete in approximately 5 minutes
- Enhanced inhibitor tolerance vs. previous generation
- Higher cDNA yield across diverse targets and species
- gDNA Digester Mix-V2: removes up to 1,000 ng residual genomic DNA
- High sensitivity: detects as low as 10 pg total RNA
- Compatible with animal, plant, and microbial RNA
Kit Components
| Component | 11155ES40 (60 rxn) | 11155ES60 (600 rxn) |
|---|---|---|
| 4× Hifair™ AdvanceFast SuperMix (11155-A) | 60 µL | 600 µL |
| gDNA Digester Mix-V2 (11155-B) | 20 µL | 200 µL |
| RNase-Free H₂O (11155-C) | 200 µL | 2 × 1 mL |
This kit completes reverse transcription in approximately 5 minutes, for a total workflow time of approximately 7 minutes including the gDNA digestion step. Standard reagents require approximately 17 minutes for the equivalent workflow — a reduction of more than 50% in hands-on and total reaction time.
gDNA Digester Mix-V2 has significantly improved genomic DNA removal efficiency, capable of effectively digesting up to 1,000 ng of residual DNA per reaction. This substantially improves performance when working with RNA preparations containing high levels of genomic DNA contamination.
This kit is validated to detect as low as 10 pg of total RNA input, enabling quantification of low-abundance targets from limited biological samples such as rare cell populations, microdissected tissue, or single cells (with appropriate pre-amplification).
Compatible with RNA from animals (mammalian, avian, fish), plants, and microorganisms. Validated across diverse RNA sources including tissue, cultured cells, blood, and plant material.
Store at −25 to −15°C for up to 1 year. The kit is shipped on dry ice to maintain reagent integrity during transit.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
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