| Field | Specification |
|---|---|
| Product Type | |
| Shipping | |
| Storage |
Hifair™ AdvanceFast One-Step RT-gDNA Digestion SuperMix for qPCR performs reverse transcription and genomic DNA removal in a single tube, streamlining workflow and significantly reducing total reaction time while increasing cDNA yield. The 4× Hifair® AdvanceFast One-Step RT SuperMix contains all essential RT reagents — add gDNA Remover Mix, RNA template, and water to initiate. Suitable for complex RNA templates and effective for low-abundance transcripts.
Features
- Single-tube simultaneous reverse transcription and gDNA elimination
- Reduced workflow time vs. two-step approaches
- Effective for complex RNA templates and low-abundance transcripts
- Stable for 12 months at −15 to −25°C
Specifications
| Parameter | Value |
|---|---|
| End Product | cDNA (First-Strand) |
| PCR Method | RT-PCR |
| Sample Type | RNA |
| Optimal Reaction Temperature | 37°C |
| Reverse Transcriptase | M-MLV (engineered) |
Kit Components
| Component | 11151ES10 (10 rxn) | 11151ES60 (100 rxn) |
|---|---|---|
| 4× Hifair® AdvanceFast One-Step RT SuperMix (11151-A) | 50 µL | 500 µL |
| gDNA Remover Mix (11151-B) | 10 µL | 100 µL |
| RNase-Free Water (11151-C) | 1 mL | 2 × 1 mL |
Reaction Setup
| Component | Volume (µL) |
|---|---|
| RNase-Free Water | To 20 |
| 4× Hifair® AdvanceFast One-Step RT SuperMix | 5 |
| gDNA Remover Mix | 1 |
| Total RNA | 10 pg – 5 µg* |
| or mRNA | 10 pg – 500 ng* |
* Do not exceed 2 µg total RNA per reaction.
The one-step format performs gDNA removal and reverse transcription in a single tube without intermediate steps or tube transfers. This significantly reduces total workflow time, minimizes RNA degradation risk during handling, and reduces the potential for cross-contamination between samples.
Total RNA input: 10 pg to 5 µg per reaction (do not exceed 2 µg for standard workflows). mRNA input: 10 pg to 500 ng. For low-expression targets, total RNA can be increased within the stated upper limit.
Shipped on dry ice. Store at −15 to −25°C for up to 12 months.
Yes. The AdvanceFast formulation is specifically designed for complex RNA templates and is validated for effective reverse transcription of low-abundance transcripts.
Both perform genomic DNA removal, but the gDNA Remover Mix in this one-step format is optimized for use in a combined RT + gDNA removal reaction at 37°C without requiring a separate digestion step. The separate 5× gDNA Digester Mix in Cat# 11141 operates in a distinct sequential digestion step before reverse transcription.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets — use code SIRNA10
20% Off Transmembrane Proteins — use code TM20
$50 Off All ELISA Kits — auto-applied at checkout (code ELISA50)
50% Off Lab Consumables + Free Shipping on orders $500+
15% Off Proteins from Trusted Suppliers — use code PROTEIN15
$99 Pipette Filler Promotion Package (reg. $236)
Save 10% on your first order of any R&D grade DENARASE® with code DENARASE10 at checkout.
