| Field | Specification |
|---|---|
| Product Type | |
| Shipping | |
| Storage |
Hifair™ III 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA Digester Plus) is a ready-made premix based on Hifair™ III Reverse Transcriptase. The third-generation enzyme offers significantly enhanced thermostability (active up to 60°C), improving reverse transcription efficiency on RNA templates with complex secondary structures. Increased template affinity makes this product effective for low-template-input applications and low-copy target genes.
Contains 5× gDNA Digester Mix for removal of residual genomic DNA from RNA templates, and 4× Hifair™ III SuperMix Plus with all required RT components (buffer, dNTPs, reverse transcriptase).
Features
- Enhanced thermostability up to 60°C — effective on structured RNA templates
- Increased template affinity: ideal for low-copy genes and limited sample inputs
- Genomic DNA removal by 5× gDNA Digester Mix
- Broad linear detection range
- Stable for 18 months at −15 to −25°C
Specifications
| Parameter | Value |
|---|---|
| End Product | cDNA (First-Strand) |
| PCR Method | RT-PCR |
| Sample Type | RNA |
| Optimal Reaction Temperature | 55–60°C |
| Reverse Transcriptase | M-MLV (engineered) |
Kit Components
| Component | 11141ES10 (10 rxn) | 11141ES60 (100 rxn) |
|---|---|---|
| RNase-Free H₂O (11141-A) | 1 mL | 2 × 1 mL |
| 5× gDNA Digester Mix (11141-B) | 30 µL | 300 µL |
| 4× Hifair™ III SuperMix Plus (11141-C) | 50 µL | 500 µL |
Hifair™ III has significantly enhanced thermostability (active up to 60°C) compared to Hifair™ II. The higher reaction temperature improves reverse transcription efficiency on RNA templates with complex secondary structures, GC-rich regions, and long mRNAs that form stable hairpin structures resistant to standard RT temperatures.
The 5× gDNA Digester Mix removes residual genomic DNA contamination from RNA preparations before reverse transcription. This eliminates false-positive signal in downstream qPCR caused by amplification of unspliced pre-mRNA or gDNA, which is particularly important for intron-spanning primer designs.
Shipped on dry ice. Store at −15 to −25°C for up to 18 months from the date of manufacture. Avoid repeated freeze-thaw cycles.
Yes. The enhanced template affinity of Hifair™ III makes this kit particularly effective for low-copy gene detection and applications with limited RNA input. The broad linear detection range supports accurate quantification across a wide dynamic range.
This kit uses an engineered M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase variant with enhanced thermostability and processivity. Unlike wild-type M-MLV RT, the engineered version retains activity at reaction temperatures up to 60°C.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
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