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Validated RNA input typically ranges from 1 pg to 100 ng total RNA per reaction, depending on target abundance. For low-abundance viral RNA, use the lowest input guidance in the datasheet. Pre-treat samples with DNase I if genomic DNA contamination is suspected, particularly for targets without intron-spanning primers.
Yes. Kits designated "(UDG Plus)" incorporate Uracil-DNA Glycosylase (UDG) to enzymatically degrade dUTP-containing PCR carryover contaminants before amplification. This is especially critical in multiplex diagnostic settings where high sample throughput increases carryover risk. UDG is inactivated by heating during the reverse transcription step.
Yes. This kit is specifically designed for multiplex one-step RT-qPCR using probe-based detection (TaqMan-compatible), allowing simultaneous detection of multiple viral RNA targets in a single tube. Internal controls can be co-amplified without reconfiguring the assay. Consult the datasheet for validated multiplex panel configurations and instrument compatibility.
This kit is compatible with major probe-based qPCR platforms including Applied Biosystems QuantStudio, Bio-Rad CFX, Roche LightCycler, and equivalent instruments with multiplex fluorescence channel capability. Ensure your instrument supports the fluorophores used in your probe panel (typically FAM, HEX/VIC, ROX, Cy5). Instrument-specific thermocycling protocols are provided in the datasheet.
Select variants of this kit (e.g., "Lyo-Ready" designations) are specifically formulated for lyophilization compatibility or room-temperature shipping to facilitate point-of-care or field deployment. Standard liquid-format kits require –20°C storage. Consult the specific product datasheet for the validated reconstitution protocol if a lyophilized format is ordered.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
