| Field | Specification |
|---|---|
| Mfr No | |
| Form | Tube, Liquid |
| Product Type | |
| Shipping | |
| Storage |
Hifair™ Universal Advanced Multiplex One-Step RT-qPCR Mix (UDG Plus) is a ready-to-use master mix designed for multiplex quantitative PCR directly from RNA templates. The reverse transcription and qPCR amplification occur in a single tube, streamlining workflow and minimizing the risk of cross-contamination. This reagent is optimized for multiplex RNA amplification
Features
- Broad compatibility: Robust performance with various primer–probe sets.
- Fast cycling: Enables rapid protocols with total run times ≤35 min.
- High specificity and sensitivity: Proprietary buffer minimizes non-specific amplification and enhances multiplex efficiency.
- dUTP/UDG system: Preventing Airborne DNA Contamination.
- Sample versatility: Tolerant to complex samples, allowing direct RT-qPCR from swab specimens without RNA purification.
Applications
- Microbial Detection, RT-qPCR Detection
- Diagnostic pathogen detection (respiratory tract, intestinal tract, etc.)
- animal pathogen detection (non-swine fever, etc.)
- food testing (microorganisms, etc.)
Specifications
| Parameter | Value |
|---|---|
| Format | Tube, Liquid |
| Sample Type | Total RNA, Viral DNA, Viral RNA, mRNA |
| Detection Method | Primer-probe |
| Inhibitor Tolerance (Simple) | High, supports direct RT-qPCR using oral swab samples with lysis buffer. |
| Instrument Compatibility | Compatible with mainstream qPCR instrument platforms. |
| Precision | CV of Ct values at high, medium, and low template concentrations ≤ 1%. |
| Linearity Range | 5 copies/reaction – 5 × 10⁵ copies/reaction; R² > 0.99; 90% ≤ Efficiency ≤ 110%. |
| Sensitivity | Detects ≤ 50 fg total human RNA. |
| Stability | 1. Freeze–thaw stability: ≥10 cycles (−20 °C freeze, normal thaw). 2. Accelerated stability: stable for 7 days at 37 °C and 14 days at 4 °C. 3. Semi–premixed system (with primers/probes): stable for 7 days at 37 °C. |
Hifair™ Universal Advanced Multiplex One-Step RT-qPCR Mix (UDG Plus) is a ready-to-use master mix designed for multiplex quantitative PCR directly from RNA templates. The reverse transcription and qPCR amplification occur in a single tube, streamlining workflow and minimizing the risk of cross-contamination. This reagent is optimized for multiplex RNA amplification
Key Features
Broad compatibility: Robust performance with various primer–probe sets. Fast cycling: Enables rapid protocols with total run times ≤35 min. High specificity and sensitivity: Proprietary buffer minimizes non-specific amplification and enhances multiplex efficiency. Anti-contamination system: dUTP/UDG system prevents carryover contamination from previous PCR products. Sample versatility: Tolerant to complex samples, allowing direct RT-qPCR from swab specimens without RNA purification.
Specifications
Format Tube, Liquid Sample Type Total RNA, Viral DNA, Viral RNA, mRNA Detection Method Primer-probe Inhibitor Tolerance (Simple) High, supports direct RT-qPCR using oral swab samples with lysis buffer. Instrument Compatibility Compatible with mainstream qPCR instrument platforms. Precision CV of Ct values at high, medium, and low template concentrations ≤ 1%. Linearity Range 5 copies/reaction – 5 × 10⁵ copies/reaction; R² > 0.99; 90% ≤ Efficiency ≤ 110%. Sensitivity Detects ≤ 50 fg total human RNA. Stability 1. Freeze–thaw stability: ≥10 cycles (−20 °C freeze, normal thaw). 2. Accelerated stability: stable for 7 days at 37 °C and 14 days at 4 °C. 3. Semi–premixed system (with primers/probes): stable for 7 days at 37 °C. Application Microbial Detection, RT-qPCR Detection; Diagnostic pathogen detection (respiratory tract, intestinal tract, etc.); animal pathogen detection (non-swine fever, etc.); food testing (microorganisms, etc.)
Kit Components
Components No. Name 16930ES60(100 T) 16930ES80(1,000 T) 16930ES92(10,000 T) 16930-A 5×Hifair™ MP Buffer 500 μL 5 mL 50 mL 16930-B Hifair™ Enzyme Mix 100 μL 1 mL 10 mL 16930-C GC-Enhancer 500μL 5 mL 50 mL [Note]: 5× Hifair™ MP Buffer is an abbreviation for 5× Hifair™ Universal Advanced Multiplex One-Step RT-qPCR Buffer. Hifair™ Enzyme Mix contains thermostable reverse transcriptase, HotStart Taq DNA polymerase, RNase inhibitor, and UDGase. Storage This product should be stored at -25℃~-15℃ for one year.
Storage & Shipping
Storage: -20°C | Shipping: Dry Ice
