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Description
Hifair™ V Multiplex One Step RT-qPCR Probe Kit (UDG Plus) is a multiplex quantitative PCR kit using RNA as the template. Reverse transcription and qPCR are performed in a single tube, simplifying the workflow and minimizing contamination risk. The kit uses a heat-stable Hifair™ V Reverse Transcriptase for efficient first-strand cDNA synthesis, and UNICON™ HotStart Taq DNA Polymerase for specific and robust amplification. It includes an optimized MP buffer with Mg²⁺, dNTPs, and additives that enhance specificity and multiplex amplification efficiency.
A dUTP/UDG system is incorporated to eliminate carryover contamination from previous PCR reactions, ensuring reliable results.
Features
- High sensitivity: The carefully optimized buffer system improves the detection sensitivity of low-concentration templates up to 250 copies/mL;
- Super stability: The product has passed real-time stability testing for 18 months;
- dUTP/UDG anti-pollution system: dUTP/ UDG anti-pollution system is introduced to degrade aerosol pollution of products efficiently, reduce false positive, and ensure authentic results;
- Support fast program: compatible with fast program, 30min can produce results.
Components
|
Components No. |
Name |
13650ES50 (50 T) |
13650ES60 (100 T) |
13650ES80 (1,000 T) |
13650ES92 (10,000 T) |
|
13650-A |
2×HifairTM V MP Buffer |
750 μL |
1.5 mL |
15 mL |
150 mL |
|
13650-B |
HifairTM V Enzyme Mix |
60 μL |
120 μL |
1.2 mL |
12 mL |
[Note]: 1) 2×HifairTM V MP Buffer is the abbreviation of HifairTM V Multiplex One Step RT-qPCR Probe Buffer, which includes dNTPs, dUTP, Mg2+, stabilizers, enhancers, and more.
2) HifairTM V Enzyme Mix mainly contains heat-resistant HifairTM V reverse transcriptase, UNICONTM HotStart Taq DNA polymeras and UDGase.
Shipping and Storage
This product should be stored at -25~-15℃ for 18 months.
Application
RT-qPCR Detection; Diagnostic pathogen detection (respiratory tract, intestinal tract, etc.); animal pathogen detection (non-swine fever, etc.); food testing (microorganisms, etc.)
Figures
1. Excellent amplification performance
Figure 1. Comparison of amplification performance of RT-qPCR kits from different brands
Amplification of gradient samples using 13650 and similar products from other suppliers in respiratory and HFMD panels showed clear S-shaped amplification curves. The Ct values of 13650 were comparable to or earlier than those of other kits, indicating superior performance.
2. Compatible with regular and fast programs
Figure 2. Test results of different reaction programs
Detection of ORF and N genes of SARS-CoV-2 pseudovirus using 13650ES under both regular and rapid programs showed no difference in amplification curve peak shape or Ct values in the 4-plex system.
3. Stability Test
Figure 3. Real-time stability testing(A) and Freeze-thaw stability(B)
Documents:
Safety Data Sheet
Manuals
Validated RNA input typically ranges from 1 pg to 100 ng total RNA per reaction, depending on target abundance. For low-abundance viral RNA, use the lowest input guidance in the datasheet. Pre-treat samples with DNase I if genomic DNA contamination is suspected, particularly for targets without intron-spanning primers.
Yes. Kits designated "(UDG Plus)" incorporate Uracil-DNA Glycosylase (UDG) to enzymatically degrade dUTP-containing PCR carryover contaminants before amplification. This is especially critical in multiplex diagnostic settings where high sample throughput increases carryover risk. UDG is inactivated by heating during the reverse transcription step.
Yes. This kit is specifically designed for multiplex one-step RT-qPCR using probe-based detection (TaqMan-compatible), allowing simultaneous detection of multiple viral RNA targets in a single tube. Internal controls can be co-amplified without reconfiguring the assay. Consult the datasheet for validated multiplex panel configurations and instrument compatibility.
This kit is compatible with major probe-based qPCR platforms including Applied Biosystems QuantStudio, Bio-Rad CFX, Roche LightCycler, and equivalent instruments with multiplex fluorescence channel capability. Ensure your instrument supports the fluorophores used in your probe panel (typically FAM, HEX/VIC, ROX, Cy5). Instrument-specific thermocycling protocols are provided in the datasheet.
Select variants of this kit (e.g., "Lyo-Ready" designations) are specifically formulated for lyophilization compatibility or room-temperature shipping to facilitate point-of-care or field deployment. Standard liquid-format kits require –20°C storage. Consult the specific product datasheet for the validated reconstitution protocol if a lyophilized format is ordered.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
