High Binding 96 Well Plates

SKU:BHU14200033
Overview
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High-binding ELISA microplate for maximum protein adsorption in sandwich and direct ELISA.
Surface High Binding
Plate Format Breakable · Strip · Solid
Well Volume 350 µl, 360 µl, 400 µl
Material Polystyrene
Binding Capacity ~ 400 ng IgG/cm2
Uniformity CV < 5 %
Blocking Standard
Available Options

Configure Your Order

Color
Clear: Recommended for colorimetric/UV readout (450 nm). Transparent bottom allows transmitted-light imaging.
White: Maximises reflectance — preferred for luminescence assays (LIA, CLIA).
Black: Minimises optical background — preferred for fluorescence assays (FIA).
Plate Format
Breakable Strips (350 µl/well): 12 individual breakable strips of 8 wells — ideal for testing variable sample numbers or running assays in multiple batches.
Strip Frame (360 µl/well): Strips on a rigid 12×8 frame that prevent misorientation — recommended for automation-assisted liquid handling and plate washers.
Solid Plate (400 µl/well): Standard solid 96-well plate — preferred for high-throughput uniform assays and large sample batches.
Order Details
Minimum order: 50 pcs. Unit: 50 pcs per unit. Storage: Room temperature. Each plate is packaged in an individual sealed barrier bag with desiccant to ensure stability until first use.
Options selector
Catalog no. Color & Well Volume Packaging
MG01F-HB8 Clear & 350 µl (Breakable Strip)
MGW01F-HB8 White & 350 µl (Breakable Strip)
MGB01F2-HB8 Black & 350 µl (Breakable Strip)
MT01F4-HB8 Clear & 360 µl (Strip Frame)
MTW01F4-HB8 White & 360 µl (Strip Frame)
MTB01F4-HB8 Black & 360 µl (Strip Frame)
MC01F-HB8 Clear & 400 µl (Solid Plate)
MCW01F-HB8 White & 400 µl (Solid Plate)
MCB01F-HB8 Black & 400 µl (Solid Plate)
Field Specification
Product Type
  • ELISA Microplate

Optimized for high-sensitivity immunoassays, Biomat High Binding 96-Well Plates provide reliable performance across various diagnostic and research applications. These plates feature a hydrophilic surface designed for passive adsorption of proteins, offering a binding capacity of 400 to 500 ng IgG/cm².

High Binding 96-Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Key Features

  • Hydrophilic High Binding Surface: Allows passive adsorption of proteins with varying hydrophilicity, and is highly selective even at low protein concentrations (< 50 ng/cm²).
  • Binding Capacity: Approximately 400 to 500 ng IgG/cm2.
  • Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
  • Wells design: flat bottom.
  • Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
  • Colors: Available in clear, white, and black polystyrene. Customizable upper rim colors upon request. Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays. Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
    • Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
    • Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
  • Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
  • Alphanumeric Coding: For easy and accurate well identification during experiments.
  • Recommended working volume: of 75 to 300 μl
  • Unit: 50 plates
  • Minimum order: 50 plates
  • Packaging: 25 per pack
  • Ready to use

Key Benefits

  • Plates Design: The mould design provides optical quality, important to reduce the background signal. The rim protects the external face of the bottom from scratches
    • The mould design provides optical quality, important to reduce the background signal.
    • The rim protects the external face of the bottom from scratches
  • Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
  • Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
  • Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
  • Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Applications

  • ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
  • Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
  • Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
  • Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.

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