| Field | Specification |
|---|---|
| Mfr No | |
| Product Type |
Streptavidin High Binding Coated Plates are ideal for capturing low molecular weight proteins or probes in ELISA assays. The high-binding surface provides a robust platform for immobilizing a wide range of biotinylated molecules, including antibodies, antigens, proteins, peptides, polysaccharides, oligonucleotides, and DNA fragments. This surface is particularly advantageous for molecules that do not adhere well through passive adsorption or that require a specific orientation for optimal binding, ensuring enhanced assay performance. Compared to standard Streptavidin Coated Plates it offers superior sensitivity and accuracy when measuring biotinylated low molecular weight molecules. This mak
Streptavidin High Binding Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.
Key Features
- High Binding Streptavidin vs. Streptavidin coated surface: particularly suited for measurement of biotinylated low molecular weight molecules.
- Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
- Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
- Wells design: flat bottom.
- Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
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Colors: Available in clear, white, and black. Customizable upper rim colors upon request. Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays. Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
- Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
- Alphanumeric Coding: For easy and accurate well identification during experiments.
- Recommended working volume: of 50 to 100 μl
- Packaging: Each coated plate is packed in a single barrier bag with desiccant
- Unit: Contains 5 plates
- Minimum order: 10 plates.
- Ready to use
Key Benefits
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Plates Design: The mould design provides optical quality, important to reduce the background signal. The rim protects the external face of the bottom from scratches
- The mould design provides optical quality, important to reduce the background signal.
- The rim protects the external face of the bottom from scratches
- Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
- Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
- Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
- Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.
Applications
- Specific and sterically oriented bond of antibodies
- Purification and detection of mouse monoclonal IgG antibodies and for purification of macaque IgG
- Highest specificity and capacity
- Retains antibody activity and orients antibody for maximum binding
- ELISA Assays: For indirect and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
- Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
- Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
- Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
- Matson RS (2023). ELISA Essentials: Surfaces, Antibodies, Enzymes, and Substrates. Methods Mol Biol. 2612:1-18. PMID: 36795356.
- Bhatt M et al. (2016). Biotin-Streptavidin Competition Mediates Sensitive Detection of Biomolecules in Enzyme Linked Immunosorbent Assay. PLoS One. 11(3):e0151154. PMID: 26954237.
- Nelson HA et al. (2022). Mitigation of Biotin Interference in Manual and Automated Immunoassays by Preconjugating Biotinylated Antibodies to the Streptavidin Surface as an Alternative to Biotin Depletion. J Appl Lab Med. 7(3):762-775. PMID: 35018420.