| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag |
| Antigen | |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | The immunogen is a mixture of various His-tagged proteins |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a monoclonal antibody produced from mouse hybridoma cells. |
| Storage | |
| Target |
Product Overview
Primary AntibodyThis antibody targets His Tag and was raised against a recombinant protein immunogen.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| ELISA | WB:1:1000-1:640000, IP:2µl-5µl |
| Western Blot (WB) | WB:1:1000-1:640000, IP:2µl-5µl |
| Immunoprecipitation (IP) | WB:1:1000-1:640000, IP:2µl-5µl |
Antibody Specifications
| Target | His Tag |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Reactivity | Species Independent |
| Purification | Protein G Purification |
| Antigen / Immunogen | The immunogen is a mixture of various His-tagged proteins |
| Clone Number | 2F4B5 |
| Production | This product is a monoclonal antibody produced from mouse hybridoma cells. |
| Form | Liquid |
| Storage Buffer | 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4. |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Alternative Names
6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag
Safety & Handling
| Target | His Tag |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Reactivity | Species Independent |
| Purification | Protein G Purification |
| Antigen/Immunogen | The immunogen is a mixture of various His-tagged proteins |
| Clone Number | 2F4B5 |
| Production | This product is a monoclonal antibody produced from mouse hybridoma cells. |
| Form | Liquid |
| Storage Buffer | 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4. |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Dilutions are starting recommendations. Titration is advised for each system.
| Application | Recommended Dilution |
|---|---|
| ELISA | WB:1:1000-1:640000, IP:2µl-5µl |
| Western Blot (WB) | WB:1:1000-1:640000, IP:2µl-5µl |
| Immunoprecipitation (IP) | WB:1:1000-1:640000, IP:2µl-5µl |
Immunogen: The immunogen is a mixture of various His-tagged proteins
Antigen Species: Species Independent
Alternative Names
6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
