| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | hist2h2l; zgc:73093; Histone H2B 3 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Zebrafish Histone H2B 3 protein (2-126AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This polyclonal anti-hist2h2l antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect hist2h2l in Zebrafish for others research.
Key elements and design rationale
- Immunogen: Recombinant Zebrafish Histone H2B 3 protein (2-126AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- HRP: Direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
Biological background
hist2h2l (also: hist2h2l, zgc:73093, Histone H2B 3) — studied in Danio rerio (Zebrafish) (Brachydanio rerio) systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current hist2h2l annotation relevant to others.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Zebrafish; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
hist2h2l is a protein studied in Zebrafish biology. This polyclonal antibody raised in Rabbit detects hist2h2l in ELISA applications, IgG isotype.
Reported reactive against Zebrafish. Immunogen from Danio rerio (Zebrafish) (Brachydanio rerio). Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
HRP: direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing hist2h2l; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
