| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Histone H3 (acetyl K9) was used as the immunogen for the Histone H3 (acetyl K9) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Histone H3 (acetyl K9) Antibody / HIST1H3A is a anti-H3 Rabbit antibody Recombinant Rabbit Monoclonal clone 32H23 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: H3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32H23, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
Histone H3 acetylated at lysine 9 plays an essential role in loosening chromatin structure, allowing transcription factors and polymerases to access DNA. Research using Histone H3 (acetyl K9) antibody has demonstrated that acetylation is mediated by histone acetyltransferases and removed by histone deacetylases. This modification serves as a signal for recruitment of chromatin remodeling complexes and transcriptional coactivators. The dynamic balance of acetylation and deacetylation at lysine 9 influences gene expression programs in development, differentiation, and disease.
Dysregulation of Histone H3 acetylation at lysine 9 has been implicated in cancer, neurological disorders, and inflammatory disease. Studies with Histone H3 (acetyl K9) antibody have shown that aberrant acetylation patterns are linked to oncogene activation or tumor suppressor silencing. In neurobiology, reduced acetylation contributes to cognitive decline, while histone deacetylase inhibitors restore acetylation and improve memory in experimental models. These findings underscore the therapeutic potential of targeting histone acetylation in disease.
Histone modifications such as H3 acetyl K9 are also important in stem cell biology and reprogramming. Research using Histone H3 (acetyl K9) antibody has confirmed that acetylation levels influence pluripotency and lineage commitment. Elevated acetylation is associated with transcriptionally open chromatin, supporting reprogramming of somatic cells to induced pluripotent stem cells. These applications highlight the broad importance of this modification across biological systems.
Histone H3 (acetyl K9) antibody is widely used in chromatin immunoprecipitation, western blotting, and immunofluorescence. Chromatin immunoprecipitation allows mapping of acetylation across genomes, western blotting detects modification-specific forms of histone H3, and immunofluorescence reveals distribution of acetylated chromatin in cells. These methods make Histone H3 (acetyl K9) antibody indispensable in epigenetics research.
By supplying validated Histone H3 (acetyl K9) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
