| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Histone H3 (tri methyl K27) was used as the immunogen for the Histone H3 (tri methyl K27) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Histone H3 (tri methyl K27) antibody / HIST1H3A is an antibody targeting Histone. Host: Rabbit. Monoclonal. Clone: 32H02. Format: Liquid. Applications: WB, IHC, ICC, IF.
Key elements and design rationale
- Target: Histone.
- Immunogen: A synthesized peptide derived from human Histone H3 (tri methyl K27) was used as the immunogen for the Histone H3 (tri….
- Species reactivity: Human, Mouse.
- Conjugate: Unconjugated (no label listed).
Biological background (from provided description)
Histone H3 (tri methyl K27) antibody detects histone H3 trimethylated at lysine 27 (H3K27me3), a hallmark of transcriptional repression and epigenetic silencing. Histone H3, encoded in part by HIST1H3A, is a core component of nucleosomes that package DNA into chromatin.
Common research applications
- WB: relative abundance and band pattern assessment using appropriate controls.
- IHC: tissue distribution assessment; interpret with matched controls.
- IF/ICC: subcellular localization imaging with proper background controls.
Notes for experimental interpretation
- Account for isoforms, paralogs, and post-translational modifications when interpreting signal.
- Support specificity with biological controls (e.g., KO/KD where available) and technical controls (e.g., secondary-only/isotype).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
