| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | H3K79ME3; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3; a; Histone H3; b; Histone H3; c; Histone H3; d; Histone H3; f; Histone H3; h; Histone H3; i; Histone H3; j; Histone H3; k; Histone H3; l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3; m; Histone H3; o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3 |
| Clonality | |
| Concentration | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthetic Peptide of Histone H3 (Tri Methyl Lys79) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
Primary AntibodyThis antibody targets HIST1H3A and detects endogenous levels of HIST1H3A protein.
Validated Applications
| Application | Dilution Guidance |
|---|---|
| Western Blot (WB) | Refer to datasheet for recommended working dilution |
Antibody Specifications
| Target | HIST1H3A |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Affinity Purification |
| Molecular Weight | ~15.3 kDa |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic Peptide of Histone H3 (Tri Methyl Lys79) |
| Formulation | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Storage | -20°C for 1 year |
| Gene ID | Human: 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968; Mouse: 319152; 15077; 15078; Rat: 291159; 100361558 |
| UniProt | P68431 (Human: P68431; Q71DI3; P84243Q6LED0; P84245) |
Scientific Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails| instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Alternative Names
H3K79ME3; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3; a; Histone H3; b; Histone H3; c; Histone H3; d; Histone H3; f; Histone H3; h; Histone H3; i; Histone H3; j; Histone H3; k; Histone H3; l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3; m; Histone H3; o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3
Safety & Handling
| Target | HIST1H3A |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Affinity Purification |
| Molecular Weight | ~15.3 kDa |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic Peptide of Histone H3 (Tri Methyl Lys79) |
| Formulation | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Storage | -20°C for 1 year |
| Gene ID | Human: 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968; Mouse: 319152; 15077; 15078; Rat: 291159; 100361558 |
| UniProt | P68431 (Human: P68431; Q71DI3; P84243Q6LED0; P84245) |
Optimized working dilutions depend on sample type and detection system. Titration is recommended.
| Application | Dilution Note |
|---|---|
| Western Blot (WB) | Refer to datasheet for recommended working dilution |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails| instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Alternative Names
H3K79ME3; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3; a; Histone H3; b; Histone H3; c; Histone H3; d; Histone H3; f; Histone H3; h; Histone H3; i; Histone H3; j; Histone H3; k; Histone H3; l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3; m; Histone H3; o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
