| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | H4; H4/n; H4F2; H4FN; FO108; HIST2H4 |
| Antigen | |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | A synthetic peptide corresponding to the amino terminus of histone H4 in which Lys20 is mono-methylated. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Product Overview
Primary AntibodyThis antibody targets HIST3H3 and was raised against a recombinant protein immunogen.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| ELISA | WB:1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
| Western Blot (WB) | WB:1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
| Immunofluorescence (IF) | WB:1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
| Immunoprecipitation (IP) | WB:1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
| Chromatin Immunoprecipitation (ChIP) | WB:1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
Antibody Specifications
| Target | HIST3H3 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Purification | Affinity Purification |
| Antigen / Immunogen | A synthetic peptide corresponding to the amino terminus of histone H4 in which Lys20 is mono-methylated. |
| Production | This product is a polyclonal antibody purified from rabbit antiserum. |
| Form | Liquid |
| Storage Buffer | PBS, 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Alternative Names
H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Safety & Handling
| Target | HIST3H3 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Purification | Affinity Purification |
| Antigen/Immunogen | A synthetic peptide corresponding to the amino terminus of histone H4 in which Lys20 is mono-methylated. |
| Production | This product is a polyclonal antibody purified from rabbit antiserum. |
| Form | Liquid |
| Storage Buffer | PBS, 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Dilutions are starting recommendations. Titration is advised for each system.
| Application | Recommended Dilution |
|---|---|
| ELISA | WB:1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
| Western Blot (WB) | WB:1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
| Immunofluorescence (IF) | WB:1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
| Immunoprecipitation (IP) | WB:1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
| Chromatin Immunoprecipitation (ChIP) | WB:1:500-1:2000, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
Immunogen: A synthetic peptide corresponding to the amino terminus of histone H4 in which Lys20 is mono-methylated.
Antigen Species: Human
Alternative Names
H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
