HiSur Protein G Beads

What it means: Magnetic Beads are polymer- or silica-matrix particles with embedded iron oxide cores that respond to an external magnetic field. They can be pulled out of solution rapidly using a permanent magnet or magnetic separation rack.

Why it matters:

• Enables hands-free wash and separation steps — no centrifuge or filter column needed.
• Particle size (50 nm–4.5 µm range) determines separation speed, surface area per mg, and suspension stability.
• Iron oxide content (18–80%) governs how fast beads respond to a magnet — higher content = faster separation.
• Surface functional groups determine which coupling chemistry and biomolecule classes can be immobilized.

How to interpret here: This product is a Magnetic Bead format. Select the size and surface chemistry variant that matches your conjugation strategy and magnetic separation setup. Check the specification sheet for magnetic content and recommended separator.

Tip: Always resuspend by bath sonication before use — magnetic beads settle during storage and may not be fully redispersed by vortexing alone.

What it means: HiSur is the high-surface-area bead series (1 µm) engineered with a porous polymer coating that provides approximately three times the surface area per bead compared to standard MonoMag 1 µm beads. This translates directly into higher functional group density and binding capacity.

Why it matters:

• ~3× surface area per bead versus MonoMag = higher antibody or protein loading per unit volume of beads.
• Enables assay development with lower total bead concentrations while maintaining equivalent capture capacity.
• ~40% iron content provides fast magnetic separation comparable to larger monodisperse formats.
• Suitable for high-sensitivity immunoassay development and diagnostic manufacturing requiring maximum ligand density.

How to interpret here: This is a HiSur series product (1 µm, single size). Select HiSur when maximum binding capacity per bead volume is the priority. Compare performance with SuperMag or MonoMag formats to confirm which best meets your binding capacity and separation speed balance.

Tip: HiSur beads are benchmarked against Sera-Mag Speedbeads in streptavidin and protein G formats, showing comparable or superior capacity in published assay comparisons.

What it means: Protein G is a bacterial surface protein from Streptococcus that binds specifically to the Fc region of IgG antibodies from a broad range of mammalian species. Covalent coupling to the particle surface exposes Protein G's IgG-binding domains for Fc-mediated antibody capture.

Why it matters:

• Binds IgG from human, mouse, rat, rabbit, bovine, goat, and other species — broad species compatibility.
• Fc-region binding leaves the antigen-binding (Fab) domains free, enabling functional antibody–antigen interaction after capture.
• Higher affinity for IgG subclasses (e.g., mouse IgG1, human IgG3) that Protein A binds poorly.
• Non-covalent binding is reversible with low-pH glycine buffer, enabling antibody recovery.

How to interpret here: This product has Protein G surface chemistry — it is pre-conjugated and ready for antibody capture. Check your antibody's species and isotype to confirm Protein G compatibility. Elution with pH 2.0–2.5 glycine buffer releases bound IgG for downstream purification.

Tip: Protein G also binds albumin from some species. If working with serum or plasma samples, a pre-clearing step with plain beads or blocking with irrelevant IgG may reduce non-specific binding.

What it means: Particle Size refers to the mean hydrodynamic diameter of the particle in suspension. Available sizes: 1 um.

Why it matters:

• Bead size affects surface area, suspension stability, and magnetic separation speed.
• Smaller particles have higher surface area per mg — more binding sites per weight of material.
• Larger particles settle faster under gravity and separate faster with a magnet.
• Select size based on your magnetic separation speed requirements, surface area needs, and assay geometry.

How to interpret here: This product is available in 1 um. Smaller sizes have more surface area per mg; larger sizes separate faster with a magnet.

Tip: Particle size is measured by DLS (dynamic light scattering) and represents the hydrodynamic diameter, which includes surface coating — actual iron core size is smaller as shown in TEM measurements in the specification sheet.

What it means: Pre-conjugated means the binding ligand (streptavidin, Protein G, or another affinity ligand) has already been covalently coupled to the particle surface during manufacturing. No additional conjugation step is needed before use in your assay.

Why it matters:

• Ready-to-use for target capture immediately after resuspension — fastest path to assay results.
• Covalent ligand attachment prevents leaching or dissociation under assay conditions.
• Quality-controlled coupling ensures consistent binding capacity lot-to-lot.
• Saves time and reduces protocol variability compared to performing your own coupling.

How to interpret here: This product is Pre-conjugated. Simply resuspend (bath sonication recommended) and use directly in your capture protocol. No EDC, NHS, or crosslinker is needed. Confirm working concentration in your specific assay format.

Tip: Store at 2–8°C without freezing. Binding activity can be verified by a simple pull-down test using a known amount of biotinylated or IgG target before committing to full assay development.

What it means: Aqueous dispersion means this product is supplied as a colloidal suspension in an aqueous buffer (typically PBS, DI water, or a proprietary storage buffer). The particles are dispersed and ready for use in water-based biological applications without any solvent exchange step.

Why it matters:

• Compatible directly with cell culture media, PBS, HEPES, and other standard biological buffers.
• No solvent exchange or phase transfer required before use in aqueous assay protocols.
• Aqueous colloidal stability maintained by surface coating (polymer, PEG, or functional group layer).
• Bath sonication before use ensures complete resuspension after storage sedimentation.

How to interpret here: This product is in Aqueous dispersion. It can be used directly in your assay buffer after resuspension. Confirm compatibility with your specific buffer conditions (ionic strength, pH, protein concentration) by checking the product specification sheet or running a small-scale stability test.

Tip: High ionic strength buffers (>500 mM) or pH extremes outside the specification range may compromise colloidal stability. Always validate in your specific matrix.

What it means: Applications list the primary experimental workflows this product has been used for: Immunoassay, Immunoprecipitation, Cell Separation.

Why it matters:

• Immunoassay: this product can directly support this workflow based on its surface chemistry and particle properties.
• Immunoprecipitation: this product can directly support this workflow based on its surface chemistry and particle properties.
• Cell Separation: this product can directly support this workflow based on its surface chemistry and particle properties.

How to interpret here: This product is suited for the listed applications. The most relevant for this product are Immunoassay, Immunoprecipitation. Review the application-specific protocol in the Documents section before beginning workflow development.

Tip: Application suitability depends on specific experimental conditions (buffer, sample matrix, target concentration). Always validate at pilot scale before full assay development.