| Field | Specification |
|---|---|
| Species |
The HK-2 cell line is a well-characterized human proximal tubular epithelial cell line derived from normal adult kidney tissue. These cells exhibit typical epithelial morphology and retain many of the biochemical and functional properties of proximal tubular cells, making them a valuable model for studying renal physiology and pathophysiology. HK-2 cells are known for their ability to perform active transport and exhibit brush border enzyme activities, which are essential for their role in renal reabsorption processes.
HK-2 cells express a range of transporters and receptors, including those for glucose, amino acids, and various ions, reflecting their role in renal filtration and reabsorption. They are also responsive to hormonal regulation, such as by parathyroid hormone and aldosterone, which influence their transport activities. Due to these characteristics, HK-2 cells are extensively used in nephrotoxicity studies, drug screening, and research on renal diseases such as acute kidney injury and chronic kidney disease.
Moreover, HK-2 cells have been utilized in studies investigating renal cell carcinoma and other kidney-related cancers. They provide a reliable in vitro system for examining cellular responses to toxic agents, oxidative stress, and hypoxia. Researchers also employ HK-2 cells to explore the molecular mechanisms underlying fibrosis and inflammation in the kidney. Overall, the HK-2 cell line is a critical tool in renal research, offering insights into both normal kidney function and disease pathogenesis.
—
- Receptors expressed: Epidermal growth factor(EGF), expressed
- Protein expression: Alkaline Phosphatase, Gamma Glutamyltranspeptidase, Leucine Aminopeptidase, Acid Phosphatase, Cytokeratin, Alpha 3, Beta 1 Integrin, Fibronectin
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets — use code SIRNA10
20% Off Transmembrane Proteins — use code TM20
$50 Off All ELISA Kits — auto-applied at checkout (code ELISA50)
50% Off Lab Consumables + Free Shipping on orders $500+
15% Off Proteins from Trusted Suppliers — use code PROTEIN15
$99 Pipette Filler Promotion Package (reg. $236)
Save 10% on your first order of any R&D grade DENARASE® with code DENARASE10 at checkout.
