| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Tissue | |
| Disease | Mast cell leukemia |
| Age | |
| Sex | |
| Growth properties | |
| Biosafety level | |
| Product format | Frozen |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
A human mast cell line was derived from HMC-1.2 cells, which express constitutively active KIT due to the presence of two oncogenic mutations: D816V and V560G. Using CRISPR technology, a site-specific mutation of V560G generated a subline, HMC-1.3, harboring a single mono-allelic D816V-KIT variant.Although HMC-1.2 and HMC-1.3 cells exhibited comparable growth in vitro, HMC-1.3 displayed significantly reduced proliferation and tumorigenicity in vivo.
Key elements and design rationale
- Model identity: HMC-1.3 Mast Cell Line (Clone 2E2) ; Not capable of histamine release (degranulation) is supplied as a tumor cell line derived from Human peripheral blood.
- Growth properties: Semi-adherent
- Growth conditions: PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. Iscove’s Modified Dulbecco’s Medium (IMDM) (TM505) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate
- Product format: Frozen, BSL-2
This cell-based model is generally used in immunology, hematology, and signaling studies. Donor/background information is available for contextual interpretation.
Biological background
Phosphorylation levels of ERK1/2, AKT, and STAT5—key pathways associated with oncogenic KIT signaling—were similar between both cell lines. However, HMC-1.3 cells showed increased sensitivity to various pharmacological inhibitors, including clinically used tyrosine kinase inhibitors (TKIs) for advanced systemic mastocytosis, as well as JAK2 and BCL2 inhibitors.These findings indicate that the additional V560G-KIT mutation in HMC-1.2 cells modulates transcriptional programs driven by D816V-KIT, conferring a survival advantage, altered drug sensitivity, and enhanced tumorigenicity. This suggests that engineered human mast cells with a single D816V-KIT mutation (HMC-1.3) may serve as an improved preclinical model for mastocytosis research and drug testing.Note: HMC do not degranulate to IgE due to the lack of the receptor. Donor/background information provided for this product: Male, 52, Mast cell leukemia.
Research relevance and current trends
- Cell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.
- Engineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.
- When metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.
Common research applications
- Cancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.
- Assay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.
- Side-by-side comparison of engineered versus parental background characteristics when relevant to the study design.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. Iscove’s Modified Dulbecco’s Medium (IMDM) (TM505) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate
- Split Ratio: 1:10-1:20
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions. Cells grow in suspension, but some cells may attach lightly to the flask. Detach cells by lightly tapping or pipetting.Change media every 3 to 4 days by splitting cultures 1:10 to 1:
- We recommend to freeze down cells from early passages, and sultures should be refreshed from earlier stock every 3 months.
- Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 2x10⁶ cells/ml. Split culture 1:10 to 1:20.
- Alternatively, collect the media with suspension cells and then detach the adherent ones with tryple express for 1-2 min. Replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
How should I handle live cells once I receive them?
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
What is your warranty or return policy?
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
