| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
HNF4α (hepatocyte nuclear factor 4 alpha) is a nuclear receptor transcription factor that functions as a homodimer and serves as a master regulator of hepatic and metabolic gene expression. In the liver it directs a large fraction of hepatic genes, including HNF1α and genes governing gluconeogenesis and lipid metabolism. HNF4α is also active in the pancreas, where it controls islet gene programs, and in the intestine, where it regulates lipid metabolism and energy expenditure. Pathogenic HNF4α mutations cause maturity-onset diabetes of the young (MODY1), and loss-of-function variants are linked to hyperinsulinemic hypoglycemia, underscoring its importance in metabolism and diabetes research.
Product Description & Applications
The HNF4α Reporter Lentivirus is a transcription-factor reporter system for sensitive fluorescent or luminescent detection of human and mouse HNF4α activity. It contains tandem repeats of consensus HNF4α DNA-binding elements, coupled to a minimal TATA-box promoter and an upstream enhancer that maximizes signal-to-noise to drive a reporter gene (d2GFP, EGFP, GFP, mCherry, RFP, or firefly luciferase). A constitutively expressed selection marker (puromycin or blasticidin) supports stable polyclonal cell line generation. Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved material, with readout by microscopy, flow cytometry, or luminometry. It is used to study HNF4α transcriptional activity in metabolism and diabetes research. Supplied as purified lentiviral particles.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the HNF4a transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
