| Field | Specification |
|---|---|
| Target | |
| Alternative names | PGK-2; PGKB; PGKPS; dJ417L20.2; phosphoglycerate kinase 1; pseudogene 2|phosphoglycerate kinase autosomal pseudogene |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The PGK2 gene encodes a testis-specific form of phosphoglycerate kinase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. the PGK2 gene lacks introns and contains characteristics of a processed gene, or 'retroposon,' including the remnants of a poly(A) tail and bounding direct repeats. The structural features were consistent with the notion that this locus arose by reverse transcriptase-mediated processing of a tailored mRNA transcript originally produced by the PGK1 gene.the unusual conservation of function in this processed PGK2 gene and its tissue-specific expression in spermatogenesis may be best explained as a compensatory response to the inactivation of the X-linked PGK1 gene in spermatogenic cells before meiosis.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate PGK2 in samples. An antibody specific for PGK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 15.6-1000 pg/mL |
| Sensitivity | 7.8 pg/mL |
| Species Reactivity | Horse (Equus caballus; Equine) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE60141HO |
| UniProt # | Q8MIF7 |
| Alternative Names | PGK-2; PGKB; PGKPS; dJ417L20.2; phosphoglycerate kinase 1; pseudogene 2|phosphoglycerate kinase autosomal pseudogene |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Horse ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for PGK2 research.
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