| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
HOXB1 is a homeobox transcription factor that helps establish anterior-posterior patterning during embryonic development, with a particularly important role in specifying segmental identity in the developing hindbrain. HOXB1 expression is tightly controlled by promoter autoregulatory elements (ARE), which contain binding sites for MEIS1-PBX-HOX complexes that direct precise spatial and temporal gene expression. Through these cooperative interactions, HOXB1 contributes to the formation of rhombomeres and the patterning of cranial motor neurons. As a member of the HOX family of developmental regulators, HOXB1 is a valuable model for studying transcriptional control of body plan formation and neural development.
Product Description & Applications
The HOXB1 Reporter Lentivirus places a reporter gene under the control of tandem HOXB1 DNA-binding elements derived from the HOXB1 promoter autoregulatory elements (ARE), providing a sensitive readout of HOXB1 transcriptional activity in human and mouse cells. Reporter options include fluorescent (GFP, EGFP, d2GFP, mCherry, RFP) and luminescent (firefly luciferase) formats, with blasticidin or puromycin selection for stable reporter cell line establishment.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Detection is possible by fluorescence microscopy, flow cytometry, or luminometry. Applications include monitoring HOXB1 activity in developmental models and studying MEIS1-PBX-HOX-mediated transcription.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the HOXB1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
