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HPAF-II is a human pancreatic adenocarcinoma cell line derived from an adult patient. This cell line is commonly used in cancer research due to its relevance in studying pancreatic cancer, a highly aggressive and lethal malignancy. HPAF-II cells exhibit epithelial morphology and are known for their ability to form tumors when xenografted into immunocompromised mice, making them a valuable model for in vivo studies of tumor growth, metastasis, and response to therapeutic interventions. Researchers often employ HPAF-II cells to investigate the molecular mechanisms underlying pancreatic cancer progression, including genetic and epigenetic alterations, signal transduction pathways, and interactions with the tumor microenvironment.
HPAF-II cells are characterized by specific genetic mutations and alterations that are frequently observed in pancreatic adenocarcinomas. These include mutations in the KRAS gene, which plays a critical role in cell signaling and proliferation, and alterations in tumor suppressor genes such as TP53 and CDKN2A. The cell line also exhibits high levels of mucin production, a feature that contributes to the aggressive nature of pancreatic tumors. Studies utilizing HPAF-II cells have provided significant insights into the biology of pancreatic cancer and have facilitated the development of potential therapeutic strategies aimed at targeting key molecular pathways involved in the disease.
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- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- doublingTime: 26 hours
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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