| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mitogen-activated protein kinase kinase kinase kinase 1; Hematopoietic progenitor kinase; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; MAP4K1; HPK1 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthetic peptide, corresponding to amino acids 365-412 of Human HPK1. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
HPK1 (L397) polyclonal antibody detects endogenous levels of HPK1 protein.
Several mammalian kinases have been identified with sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include HPK1, KHS, GLK, NIK, YSK1, Krs-1, Krs-2 and GC kinase. HPK1 (hematopoietic progenitor kinase 1), like many other STE20-like kinases, specifically activates the JNK signaling pathway.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | 1:500~1:1000 |
Product Specifications
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Isotype | IgG |
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 97 kDa |
| Modification | Unmodified |
| UniProt / Swiss-Prot | Q92918 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Signaling Pathway(s) | SAPK JNK Signaling Cascades |
Immunogen / Antigen
Synthetic peptide, corresponding to amino acids 365-412 of Human HPK1.
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for WB. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Mouse, Rat. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Rabbit. For indirect detection (WB, IHC, IF), select an anti-Rabbit secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Synthetic peptide, corresponding to amino acids 365-412 of Human HPK1.
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Mouse, Rat samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
