| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
HRH1 encodes the histamine H1 receptor, a G protein-coupled receptor that mediates many physiological effects of histamine. Coupled mainly to Gq/11 proteins, H1 receptor activation triggers phospholipase C signaling, intracellular calcium mobilization, and downstream pathways that regulate smooth muscle contraction, vascular permeability, and neuronal activity. The H1 receptor is central to allergic and inflammatory responses, where it drives bronchoconstriction, vasodilation, and itch, and it also contributes to wakefulness and neurotransmission in the central nervous system. As the molecular target of antihistamine drugs, HRH1 is widely studied in immunology, pharmacology, and neuroscience research.
Product Description & Applications
The HRH1 ORF cDNA Lentivirus enables stable overexpression of the human histamine H1 receptor (HRH1) open reading frame in mammalian cells. A CMV promoter drives the HRH1 ORF, with constructs that may include a C-terminal tag, a fluorescent reporter such as EGFP or mCherry, and an antibiotic selection marker, linked by self-cleaving peptide sequences depending on configuration. The third-generation lentiviral vector provides high gene expression and reliable genome integration for rapid stable cell line generation. Applications include studying H1 receptor signaling, GPCR pharmacology, antihistamine drug action, and histamine-driven inflammatory responses. Particles transduce difficult-to-transfect cells, including primary and thawed cells; cloning accuracy is sequence-verified and protein expression validated by transient transfection.
About This Product
This ORF cDNA lentivirus enables stable overexpression of HRH1 (NCBI Accession: NM_001098213) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (EGFP, GFP, mCherry, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.
Ultra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
