| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Rabbit IgG(H+L) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from goat antiserum. |
| Storage | |
| Target |
Overview
This is a polyclonal anti-IgG antibody raised in Goat, conjugated with HRP, with confirmed utility in ELISA, WB. It is designed to detect IgG protein in Rabbit and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Rabbit IgG(H+L) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Goat): Requires anti-goat-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
- HRP conjugate: Direct detection with TMB/OPD or ECL substrates. Quench endogenous peroxidase before use in tissue assays.
Biological background
IgG is a protein target studied in Rabbit systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of IgG biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Goat) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Rabbit. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from goat antiserum.
IgG is a protein target in Rabbit biology. This polyclonal antibody raised in Goat is designed to detect IgG in ELISA, WB applications, with IgG isotype.
Reported reactive against Rabbit. Immunogen derived from Rabbit. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, WB. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
HRP enables direct TMB/ECL detection without secondary. Quench endogenous peroxidase in tissue assays.
(1) Isotype control — IgG from Goat at matching concentration; (2) Positive control — known IgG-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
