HRP, Rabbit Anti-Goat IgG

Reactivity: Goat

What it means: This secondary antibody is designed to recognize goat primary antibodies (e.g., goat IgG). It binds to the primary antibody and enables detection through its conjugate (enzyme or fluorophore).

Why it matters: Choose “Goat” reactivity when your primary antibody is raised in goat. Note that in goat-derived samples, endogenous goat immunoglobulins may contribute to background in certain applications.

Host: Rabbit

What it means: “Host” refers to the animal in which the secondary antibody was produced. Host: Rabbit means the antibody was generated by immunizing a rabbit, and the resulting rabbit antibodies were collected and purified to make this secondary antibody.

Why it matters: The host species can affect background and cross-reactivity. For best results, avoid using a rabbit-host secondary in workflows where rabbit immunoglobulins are present (e.g., rabbit primary antibodies in the same assay, rabbit serum-based blocking, or rabbit-derived samples), unless the protocol includes appropriate blocking and controls.

This secondary antibody is tagged with HRP so you can visualize/detect your target using an HRP substrate.

What it does: HRP acts as a reporter enzyme. After the secondary binds to your primary antibody, you add an HRP substrate (commonly TMB for ELISA or ECL chemiluminescent substrates for Western blot), and HRP catalyzes a reaction that produces a detectable signal (color or light).

Why it matters: HRP-conjugated secondaries are widely used because they give strong signal, are cost-effective, and work well for WB, ELISA, and IHC (with appropriate substrates).

Application(s): WB, IHC-P, ELISA

WB (Western Blot): Used as a secondary antibody to bind the primary antibody on membranes after SDS-PAGE transfer, enabling detection via enzyme- or fluorophore-based signal readout (depending on the conjugate).

IHC-P (Immunohistochemistry—Paraffin): Used as a secondary antibody to detect primary antibodies bound to antigens in paraffin-embedded (FFPE) tissue sections, enabling visualization after standard deparaffinization and antigen retrieval with the appropriate detection reagent.

ELISA: Used as a secondary antibody in plate-based assays to bind the primary antibody and generate quantitative or semi-quantitative signal through its conjugate-based detection system.

Isotype: Rabbit IgG

What it means: This secondary antibody is a rabbit-derived immunoglobulin G (IgG). In other words, it is an IgG antibody produced in rabbit and purified for use as a secondary reagent.

Why it matters: “Rabbit IgG” is mainly useful for understanding the species origin and antibody class of the secondary antibody:

• Species origin (rabbit): This tells you the secondary itself is made in rabbit. If your sample contains rabbit immunoglobulins (e.g., rabbit serum/plasma, rabbit tissue, or you are working in rabbit models), endogenous rabbit IgG can contribute to background. In such cases, additional blocking or an alternative secondary/detection strategy may be needed.
• Antibody class (IgG): IgG is the most commonly used antibody class for secondary reagents and typically provides strong, reliable performance in routine detection workflows.
• Detection and capture compatibility: Many detection/capture reagents are designed to bind IgG (e.g., anti-rabbit IgG reagents and, in some workflows, Protein A/G). Knowing it is rabbit IgG helps confirm compatibility when extra capture/detection steps are used.
• Controls: If you are using anti-rabbit detection reagents, “rabbit IgG” helps when selecting appropriate negative controls to evaluate non-specific binding from rabbit-derived antibodies.