| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
The heat shock element (HSE) is a conserved promoter motif that mediates the heat shock response, the cell's protective program against proteotoxic stress. Heat, oxidative stress, and protein-damaging conditions activate heat shock factor 1 (HSF1), which trimerizes, accumulates in the nucleus, and binds HSEs to induce molecular chaperones such as HSP70 and HSP90. These chaperones refold or clear misfolded proteins and restore proteostasis. HSF1 also influences cell survival, metabolism, and stress adaptation, and its activity is frequently elevated in cancer to support malignant proteostasis. HSE-driven reporters provide a direct readout of HSF1 activation and heat shock response signaling.
Product Description & Applications
The HSE Reporter Lentivirus places a reporter gene under the control of tandem heat shock elements, providing a sensitive readout of HSF1 activation and heat shock response pathway activity in human and mouse cells. Reporter options include fluorescent (GFP, RFP) and luminescent (firefly and Renilla luciferase) formats, with puromycin or blasticidin selection for rapid generation of stable reporter cell lines. Signal can be measured by fluorescence microscopy, flow cytometry, or luminometry.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring proteotoxic stress responses, studying proteostasis in cancer, and screening compounds that modulate HSF1 activity.
About This Product
This reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Heat shock response pathway (HSF-1) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
