| Field | Specification |
|---|---|
| Target | |
| Alternative names | HSF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSTF1, HSF 1 |
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| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant mouse HSF1 protein |
| Purification | |
| Cellular localization | |
| Concentration | |
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| Shipping | |
| Catalog no. (Mfr.) | |
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Heat Shock Factor 1 (HSF1) is a key transcriptional regulator that orchestrates the cellular response to proteotoxic stress by activating genes involved in protein folding, degradation, and quality control. As the primary mediator of the heat shock response, HSF1 binds to heat shock elements (HSEs) in the promoters of heat shock protein (HSP) genes, driving the expression of molecular chaperones essential for maintaining proteostasis.
Structurally, HSF1 contains a conserved N-terminal DNA-binding domain and adjacent hydrophobic repeats critical for trimerization and activation. A second hydrophobic region near the C-terminus suppresses trimer formation under non-stress conditions. In unstressed cells, HSF1 is diffusely localized in the cytoplasm and nucleus. Upon exposure to stressors—such as oxidative damage, heat, or protein misfolding—HSF1 rapidly translocates to nuclear stress granules, initiating a transcriptional program that restores protein homeostasis.
In the context of neurodegenerative diseases, HSF1 plays a pivotal role in mitigating the toxic accumulation of misfolded proteins, a hallmark of disorders such as Alzheimer’s, Parkinson’s, and Huntington’s disease. By enhancing the expression of protective HSPs, HSF1 supports neuronal survival and function under chronic stress conditions. Its dynamic regulation and neuroprotective potential make HSF1 a compelling therapeutic target in neuroscience and neurodegeneration research.
1 µg/ml of SMC-476 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
Cite this product varies by variant:
- SMC-476D — Size: 100 ug: HSF1 Antibody (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-476D, RRID: AB_2702279)
- SMC-476D-A390 — Size: 100 ug: HSF1 Antibody: ATTO 390 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-476D-A390, RRID: AB_2702280)
- SMC-476D-A488 — Size: 100 ug: HSF1 Antibody: ATTO 488 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-476D-A488, RRID: AB_2702281)
- SMC-476D-A594 — Size: 100 ug: HSF1 Antibody: ATTO 594 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-476D-A594, RRID: AB_2702283)
- SMC-476D-APC — Size: 100 ug: HSF1 Antibody: APC (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-476D-APC, RRID: AB_2702289)
- SMC-476D-BI — Size: 100 ug: HSF1 Antibody: Biotin (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-476D-BI, RRID: AB_2702290)
- SMC-476D-FITC — Size: 100 ug: HSF1 Antibody: FITC (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-476D-FITC, RRID: AB_2702291)
- SMC-476D-HRP — Size: 100 ug: HSF1 Antibody: HRP (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-476D-HRP, RRID: AB_2702292)
- SMC-476D-PCP — Size: 100 ug: HSF1 Antibody: PerCP (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-476D-PCP, RRID: AB_2702294)
- SMC-476D-RPE — Size: 100 ug: HSF1 Antibody: RPE (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-476D-RPE, RRID: AB_2702295)
- SMC-476S — Size: 12 ug: HSF1 Antibody (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-476S, RRID: AB_2702279)
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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