| Field | Specification |
|---|---|
| Mfr No | |
| Assay Type | |
| Product Type | |
| Sample Type(s) | serum, urine |
| Species | |
| Storage | |
| Target |
Assay Principle
7-Dehydrocholesterol (7-DHC) is a cholesterol precursor in the serum and is photochemically converted to vitamin D3 in the skin. Its functioning serves as a provitamin-D3 to vitamin D in the body upon exposure to ultraviolet B (UVB) radiation from sunlight. A deficiency in 7-DHC can lead to various health issues, such as increased risk of osteoporosis, cancer and higher susceptibility to infections. The Tribo 7-DHC ELISA Kit can quickly, sensitively, and accurately determine the presence of 7-DHC in CSF, urine, serum and other biological samples.
Intended Use The Tribo 7-DHC ELISA Kit utilizes competitive ELISA for the quantitative and qualitative analysis of 7-DHC in biological samples. The limit of detection (LOD) of 7-DHC in ELISA Kit is 0.2ng/ml.
Typical Data This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Human 7-DHC ELISA Kit is a Competitive ELISA for the quantitative determination of 7-DHC. In this competitive ELISA, sample analyte competes with a labeled competitor for a limited number of antibody binding sites, so signal is inversely proportional to analyte concentration.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | 7-DHC |
|---|---|
| Species | Human |
| Sample Type | serum, urine |
| Assay Type | Competitive |
| Test Principle | Competitive ELISA |
| Storage | Store the kit at 4°C and the Standard and Detection reagents at -20°C, respectively. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for serum, urine. Other matrices may require additional in-lab validation.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this competitive ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
