| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiopoietin-2;ANG-2;ANGPT2; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: Y19-F496 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Saliva, Milk |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human angiopoietin-2 (gene ANGPT2) in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and human milk. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Angiopoietin-2 (ANGPT2) is a TIE2 (TEK) ligand that competes with angiopoietin-1 and often acts as a context-dependent antagonist. It loosens vessel stability, promoting sprouting when VEGF is present and vessel regression when it is not, and it is widely studied in tumor angiogenesis, inflammation and vascular leakage.
Kit Components
| Anti-Human ANGPT2 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human ANGPT2 Standard | 2 vials, 10 ng/tube |
| Human ANGPT2 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and human milk |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human ANGPT2 (Tyr19–Phe496), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.3–7.0% (3 samples, n = 16 each) |
| Inter-assay CV | 6.3–7.2% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured about 2 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.023; 156 = 0.112; 312 = 0.198; 625 = 0.318; 1250 = 0.611; 2500 = 1.1; 5000 = 1.696; 10000 = 2.017. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.023 | 0.112 | 0.198 | 0.318 | 0.611 | 1.1 | 1.696 | 2.017 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 327 | 1634 | 5900 | 341 | 1769 | 5438 |
| Standard deviation | 17.33 | 114.38 | 354 | 23.52 | 127.36 | 342.59 |
| CV (%) | 5.3% | 7% | 6% | 6.9% | 7.2% | 6.3% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Alison Cash et al. (2023) Endothelial deletion of EPH receptor A4 alters single-cell profile and Tie2/Akap12 signaling to preserve blood–brain barrier integrity. Proceedings of the National Academy of Sciences of the United States of America. 10.1073/pnas.2204700120
Zhang Caifeng et al. (2024) Atg5-deficient mesenchymal stem cells protect against non-alcoholic fatty liver by accelerating hepatocyte growth factor secretion. Cell Communication and Signaling. 10.1186/s12964-024-01950-x
Michele Dei Cas et al. (2021) Plasma Lipid Profiling Contributes to Untangle the Complexity of Moyamoya Arteriopathy. International Journal of Molecular Sciences. 10.3390/ijms222413410
Gemma Gorla et al. (2023) Increase of Circulating Endothelial Progenitor Cells and Released Angiogenic Factors in Children with Moyamoya Arteriopathy. International Journal of Molecular Sciences. 10.3390/ijms24021233
Jiaojiao Chen et al. (2024) Geniposide alleviates imiquimod-induced psoriasis-like skin lesions in mice via inhibition of angiogenesis. International Immunopharmacology. 10.1016/j.intimp.2024.111923
Gemma Gorla et al. (2024) Angiopoietin-2 associates with poor prognosis in Moyamoya angiopathy. Annals of Clinical and Translational Neurology. 10.1002/acn3.52076
You Hua et al. (2015) Vascular Endothelial Growth Factor Enhanced the Angiogenesis Response of Human Umbilical Cord-Derived Mesenchymal Stromal Cells in a Rat Model of Radiation Myelopathy. Neurochemical Research. 10.1007/s11064-015-1684-0
Peng Zheng et al. (2017) Simvastatin ameliorates graft-vs-host disease by regulating angiopoietin-1 and angiopoietin-2 in a murine model. Leukemia Research. 10.1016/j.leukres.2017.01.017
Qiao-Ying You et al. (2014) Effects of laser photocoagulation on serum angiopoietin-1, angiopoietin-2, angiopoietin-1/angiopoietin-2 ratio, and soluble angiopoietin receptor Tie-2 levels in type 2 diabetic patients with proliferative diabetic retinopathy. International Journal of Ophthalmology. 10.3980/j.issn.2222-3959.2014.04.11
