| Field | Specification |
|---|---|
| Target | |
| Alternative names | ACEH; DKFZp434A014; ACE-related carboxypeptidase|angiotensin I converting enzyme 2|angiotensin converting enzyme-like protein |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
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Scientific Background
ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin ,or the conversion of angiotensin II to angiotensin. ACE 2 has direct effects on cardiac function, and is expressed predominantly in vascular endothelial cells of the heart and the kidneys.By EST database searching for sequences showing homology to the zinc metalloprotease angiotensin-I converting enzyme (ACE) and by screening a human lymphoma cDNA library, Tipnis et al. (2000) cloned a full-length ACE2 cDNA, which they called ACEH, encoding a deduced 805-amino acid protein that shares approximately 40% identity with the N- and C-terminal domains of ACE. ACE2 contains a potential 17-amino acid N-terminal signal peptide and a putative 22-amino acid C-terminal membrane anchor.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate ACE2 in samples. An antibody specific for ACE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 15.6-1000 pg/mL |
| Sensitivity | 6.4 pg/mL |
| Species Reactivity | Human (Homo sapiens) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE23396HU |
| UniProt # | Q9BYF1 |
| Alternative Names | ACEH; DKFZp434A014; ACE-related carboxypeptidase|angiotensin I converting enzyme 2|angiotensin converting enzyme-like protein |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Human ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for ACE2 research.
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