Human APRIL Protein, hFc Tag

What it means: Mammalian (HEK293) — this protein is produced in Mammalian (HEK293), enabling human-like glycosylation, correct disulfide bond formation, and proper protein folding.

Why it matters:

• HEK293 mammalian expression is preferred for transmembrane proteins, Fc-fusion proteins, and proteins requiring N-glycosylation for correct folding and binding activity.
• Mammalian-expressed proteins are particularly important for binding interaction studies (SPR/BLI) and antibody epitope validation where native conformation is critical.

How to interpret here: Produced in Mammalian (HEK293). Verify that Mammalian (HEK293) glycosylation is compatible with your downstream antibody characterization or binding assay requirements.

What it means: N-Human Fc Tag — this affinity tag is used for protein purification and can serve as a capture or detection handle in assays.

Why it matters:

• Human Fc tag (hFc) enables protein A/G-based purification and increases avidity in bivalent binding assays; Fc-tagged proteins also have extended serum half-life for in vivo studies.
• His-tag enables IMAC capture and is compatible with anti-His antibodies for direct ELISA or Western blot detection.
• Strep-tag enables high-affinity streptavidin capture for SPR/BLI sensor chip immobilisation.
• Flag-tag provides a compact epitope ideal for co-IP and pull-down assays.

How to interpret here: Use N-Human Fc Tag for purification, capture, or detection. Note tag position (N- vs C-terminal) may affect domain accessibility.

What it means: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. — protein purity confirmed by SDS-PAGE and Coomassie staining under reducing conditions.

Why it matters:

• High purity (>95%) is critical for binding kinetics (SPR/BLI) where contaminants can introduce artefactual binding signals.
• For antibody-antigen binding assays and ELISA standards, purity directly determines assay linearity and sensitivity.

How to interpret here: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. by SDS-PAGE. Lot-specific gel images and QC data are provided with each shipment via Certificate of Analysis.

What it means: In Stock — estimated availability from the manufacturer for this product.

Why it matters:

• Lead time directly affects experimental scheduling, particularly for research programs with milestone deliverables or grant deadlines.
• Some transmembrane proteins and nanodisc preparations require custom expression runs, resulting in longer lead times.

How to interpret here: In Stock. Contact BioHippo to confirm current stock and place time-sensitive orders. Rush orders may be available for select products.

What it means: Predicted molecular mass of The protein has a predicted molecular mass of 42.5 kDa after removal of the signal peptide. The apparent molecular mass of hFc-APRIL is approximately 35-55 kDa due to glycosylation. after signal peptide removal, with the apparent MW observed by SDS-PAGE.

Why it matters:

• The apparent MW on SDS-PAGE may differ from predicted MW due to glycosylation (especially for HEK293-expressed proteins) or anomalous SDS binding.
• Use the apparent MW to confirm correct protein identity and purity in your Western Blot or SDS-PAGE QC step.

How to interpret here: Expected band at approximately The protein has a predicted molecular mass of 42.5 kDa after removal of the signal peptide. The apparent molecular mass of hFc-APRIL is approximately 35-55 kDa due to glycosylation. on reducing SDS-PAGE. For glycoproteins, the apparent MW may be higher than predicted due to N-linked glycosylation in the HEK293 expression system.

What it means: Ala105-Leu250 — this protein construct spans the indicated amino acid region of the target protein.

Why it matters:

• The expressed region determines which domains are present in the preparation, including the receptor-binding domain, ligand-binding site, or epitope region.
• For SARS-CoV-2 and viral proteins, the expressed variant (mutation codes) directly determines cross-reactivity with antibody panels and neutralisation assay compatibility.

How to interpret here: Confirm the region Ala105-Leu250 includes your target domain or epitope before ordering. Cross-reference with UniProt for domain boundary annotations.

What it means: UniProt accession O75888 is the canonical identifier for this protein sequence in the Universal Protein Resource.

Why it matters:

• UniProt provides authoritative sequence data, domain maps, known SNPs/mutations, post-translational modification sites, and literature links.
• Essential for verifying the expressed region includes your target domain, and for selecting appropriate antibodies or reagents for validation.

How to interpret here: Visit uniprot.org/O75888 to confirm sequence identity, domain architecture, and known ligand/binding partner information.