| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | VMD2L3 |
| Conjugate | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Reconstitution | |
| Shipping | |
| Storage | |
| Target | |
| UniProt # |
Scientific Background
BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries.
Product Description
Mammalian (HEK293) Expression | Transmembrane ProteinRecombinant BEST3 Human protein is produced in Mammalian (HEK293) expression system and supplied as lyophilized powder for stable long-term storage. Suitable for binding assays, SPR/BLI interaction studies, antibody development, ELISA development, and drug discovery applications.
Protein Specifications
| UniProt ID | Q8N1M1 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Affinity Tag | C-Flag&Strep Tag |
| Molecular Weight | 76.1 kDa |
| Formulation | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% –… |
| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for… |
| Delivery | 6~8weeks |
| Protein Families | Ion Channels: Other |
Safety & Handling
Research use only
This protein was produced in Mammalian (HEK293). Mammalian expression enables human-like glycosylation, correct disulfide bond formation, and proper protein folding — all critical for proteins used in binding interaction studies (SPR/BLI) and antibody characterisation. For transmembrane proteins expressed as ectodomains or nanodisc preparations, the expression system directly affects binding conformation and assay performance.
The expressed amino acid region is specified in the protein specifications table. Cross-reference with the UniProt entry (Q8N1M1) to confirm the expressed region includes your domain of interest — receptor-binding domain, extracellular domain, or specific epitope — before ordering.
>95% purity by SDS-PAGE and Coomassie staining. A gel image showing the protein band is provided with each lot on the Certificate of Analysis. This high-purity specification is suitable for SPR/BLI kinetics measurements, ELISA standard curve development, and antibody epitope binding studies.
Please refer to the Certificate of Analysis (CoA) for reconstitution instructions for your specific lot. Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (A Prepare single-use working aliquots after reconstitution to avoid repeated freeze-thaw cycles. Lyophilized protein is stable for 12 months at −80°C from the date of receipt.
6–8 weeks lead time required; contact biohippo for current stock status. Contact BioHippo to confirm current availability and place time-sensitive orders. For large-scale or bulk requirements, extended lead times may apply for custom production runs.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Alternative tag formats: Different Fc isotypes (hFc vs mFc), His-tag, or biotinylated variants may be available upon request.
- Biotinylated proteins: Site-specific biotinylation for SPR/BLI streptavidin-coated chip immobilisation.
- Nanodisc preparations: Full-length transmembrane protein in synthetic nanodisc format for soluble binding studies.
- Bulk quantities: mg-scale production for high-throughput screening.
- Custom species variants: Mouse, rat, or non-human primate orthologs may be available.
Contact BioHippo customer support for custom requirements.
