| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin; INS |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: F25-N110 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA kit quantifies canine, human and porcine insulin in cell culture supernatants, serum and plasma (heparin, EDTA). The target is captured on a pre-coated plate, detected with an enzyme-linked system and read at 450 nm against a standard curve.
Insulin is a peptide hormone secreted by pancreatic beta cells that lowers blood glucose. It is made as proinsulin and processed into A and B chains joined by disulfide bonds, releasing C-peptide. By activating the insulin receptor, it promotes glucose uptake in muscle and fat and glycogen and lipid synthesis in the liver, making it central to diabetes and metabolism research.
Kit Components
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Performance Data
| Species | Canine, Human, Porcine |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA) |
| Assay range | 15.6 pmol/L - 1,000 pmol/L |
| Sensitivity | <2 pmol/L |
| Standard | Recombinant canine, human and porcine INS (Phe25–Asn110), expressed in E. coli |
| Cross-reactivity | No significant cross-reactivity or interference with INS analogs in supplier testing; untested analogs cannot be excluded. |
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pmol/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.004 | 0.086 | 0.184 | 0.294 | 0.56 | 0.989 | 1.621 | 2.158 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pmol/L) | 28 | 93 | 474 | 25 | 118 | 274 |
| Standard deviation | 1.53 | 2.72 | 10.57 | 1.14 | 5.17 | 21.81 |
| CV(%) | 5.5% | 2.9% | 2.2% | 4.5% | 4.4% | 8% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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