| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dipeptidyl peptidase 1; Cathepsin C; Cathepsin J; Dipeptidyl peptidase I; DPP-I; DPPI; Dipeptidyl transferase; CTSC; CPPI |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: D25-L463 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human dipeptidyl peptidase 1 (gene CTSC) in cell culture supernatants, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Cathepsin C (CTSC, also called dipeptidyl peptidase I) is a lysosomal cysteine protease that removes dipeptides from the N-terminus of its substrates. It activates serine proteases stored in immune cell granules, such as neutrophil elastase, cathepsin G and the granzymes. Loss of CTSC causes Papillon-Lefèvre syndrome, and it is studied in neutrophil and cytotoxic lymphocyte biology.
Kit Components
| Anti-Human CTSC Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human CTSC Standard | 2 vials, 10 ng/tube |
| Human CTSC Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <75 pg/mL |
| Standard | Recombinant human CTSC (Asp25–Leu463), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 2.8–7.5% (3 samples, n = 16 each) |
| Inter-assay CV | 2.5–5.8% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured 1616–2624 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.036; 156 = 0.095; 312 = 0.155; 625 = 0.261; 1250 = 0.455; 2500 = 0.816; 5000 = 1.321; 10000 = 2.119. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.036 | 0.095 | 0.155 | 0.261 | 0.455 | 0.816 | 1.321 | 2.119 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 163 | 470 | 3588 | 258 | 505 | 3776 |
| Standard deviation | 4.56 | 32.15 | 267.66 | 10.06 | 12.47 | 219.01 |
| CV(%) | 2.8% | 6.8% | 7.5% | 3.9% | 2.5% | 5.8% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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