| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C motif chemokine 17;CC chemokine TARC;Small-inducible cytokine A17;Thymus and activation-regulated chemokine;CCL17;SCYA17, TARC; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: A24-S94 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human C-C motif chemokine 17 (gene CCL17) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
CCL17 (also called TARC) is a C-C chemokine that binds the receptor CCR4 and preferentially attracts Th2 cells and regulatory T cells. It is produced by dendritic cells and other cells and is elevated in allergic diseases such as atopic dermatitis, where it is commonly measured as a disease activity marker.
Kit Components
| Anti-Human CCL17 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human CCL17 Standard | 2 vials, 10 ng/tube |
| Human CCL17 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant human CCL17 (Ala24–Ser94), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.2–5.7% (3 samples, n = 16 each) |
| Inter-assay CV | 4.8–6.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum samples measured 41–678 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.052; 31.2 = 0.13; 62.5 = 0.189; 125 = 0.274; 250 = 0.441; 500 = 0.764; 1000 = 1.268; 2000 = 1.967. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.052 | 0.13 | 0.189 | 0.274 | 0.441 | 0.764 | 1.268 | 1.967 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 62 | 251 | 939 | 56 | 234 | 931 |
| Standard deviation | 2.6 | 10.79 | 53.52 | 2.68 | 12.16 | 62.37 |
| CV (%) | 4.2% | 4.3% | 5.7% | 4.8% | 5.2% | 6.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Liu Tingting et al. (2024) Single-cell transcriptomics analysis of bullous pemphigoid unveils immune-stromal crosstalk in type 2 inflammatory disease. Nature Communications. 10.1038/s41467-024-50283-3
Jia Bi et al. (2017) RETRACTED: miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα. Molecular Therapy-Nucleic Acids. 10.1038/mtna.2016.71
Leonard Blum et al. (2020) Natural antiviral compound silvestrol modulates human monocyte-derived macrophages and dendritic cells. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.15360
Bao-Feng Wang et al. (2016) Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China. International Forum of Allergy & Rhinology. 10.1002/alr.21799
Hong-Yang Du et al. (2016) The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway. Journal of Interferon and Cytokine Research. 10.1089/jir.2015.0159
O. Bilgic et al. (2015) Serum cytokine and chemokine profiles in patients with alopecia areata. Journal of Dermatological Treatment. 10.3109/09546634.2015.1093591
