| Field | Specification |
|---|---|
| Alternative Names | PSGL-1;PSGL1;CD162;SELPLG;Selectin P ligand |
| Conjugate | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Storage | |
| Target | |
| UniProt # |
Scientific Background
This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity.
Product Description
Mammalian (HEK293) Expression | Recombinant ProteinRecombinant SELPLG Human protein is produced in Mammalian (HEK293) expression system and supplied as lyophilized powder for stable long-term storage. Suitable for binding assays, SPR/BLI interaction studies, antibody development, ELISA development, and drug discovery applications.
Protein Specifications
| UniProt ID | Q14242 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Molecular Characterization | CD162(Leu18-Cys320) 6×His tag |
| Amino Acids / Region | Leu18-Cys320 |
| Affinity Tag | C-6×His Tag |
| Molecular Weight | 31.4 kDa |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyo… |
| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for… |
| Delivery | In Stock |
Safety & Handling
Research use only
This protein was produced in Mammalian (HEK293). Mammalian expression enables human-like glycosylation, correct disulfide bond formation, and proper protein folding — all critical for proteins used in binding interaction studies (SPR/BLI) and antibody characterisation. For transmembrane proteins expressed as ectodomains or nanodisc preparations, the expression system directly affects binding conformation and assay performance.
The molecular characterisation of this construct is: CD162(Leu18-Cys320) 6×His tag. Cross-reference with the UniProt entry (Q14242) to confirm the expressed region includes your domain of interest — receptor-binding domain, extracellular domain, or specific epitope — before ordering.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.. A gel image showing the protein band is provided with each lot on the Certificate of Analysis. This high-purity specification is suitable for SPR/BLI kinetics measurements, ELISA standard curve development, and antibody epitope binding studies.
Please refer to the Certificate of Analysis (CoA) for reconstitution instructions for your specific lot. Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (A Prepare single-use working aliquots after reconstitution to avoid repeated freeze-thaw cycles. Lyophilized protein is stable for 12 months at −80°C from the date of receipt.
Options listed in “availability content”; other statuses may take longer. Contact BioHippo to confirm current availability and place time-sensitive orders. For large-scale or bulk requirements, extended lead times may apply for custom production runs.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Alternative tag formats: Different Fc isotypes (hFc vs mFc), His-tag, or biotinylated variants may be available upon request.
- Biotinylated proteins: Site-specific biotinylation for SPR/BLI streptavidin-coated chip immobilisation.
- Nanodisc preparations: Full-length transmembrane protein in synthetic nanodisc format for soluble binding studies.
- Bulk quantities: mg-scale production for high-throughput screening.
- Custom species variants: Mouse, rat, or non-human primate orthologs may be available.
Contact BioHippo customer support for custom requirements.
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