Human CD171/L1CAM ELISA Kit

What it means: Assay Type describes how the kit generates its signal and whether it reports concentrations. For this kit the assay type is Quantitative, so each well is read against the standard curve. Standards and samples are run on the same plate and compared through the standard curve.

Why it matters:

• Each plate needs its own standard series.
• Knowing the format helps when choosing curve-fitting settings in the analysis software.

How to interpret here: For this Quantitative assay, run the full standard series on every plate and calculate sample values from that plate's own curve; a four-parameter logistic fit is a common choice.

Tip: Run standards and samples in duplicate and include a blank on every plate.

What it means: Sample Type lists the sample matrices recommended for this kit: Serum, Plasma. Each matrix can affect background and recovery differently, so the sample type should match the matrix used in the study.

Why it matters:

• Lipids, hemoglobin and anticoagulants in a sample can change background and recovery.
• Following the recommended dilution helps keep readings within the standard curve.
• Collection, storage and freeze-thaw history can affect results, so handle all samples in a study the same way.

How to interpret here: For Serum, Plasma, follow the sample handling and dilution guidance on the datasheet; check dilution linearity and spike recovery before testing many samples in a new matrix.

Tip: For Serum, Plasma, compare results only between samples of the same matrix and avoid repeated freeze-thaw cycles.

What it means: Detection Range is the span of the standard curve within which concentrations can be calculated reliably. For this kit the detection range is 1.56 - 100 ng/mL. Values outside this span should not be reported directly.

Why it matters:

• Samples above the top standard must be diluted and run again.
• Readings near either end of the curve carry more error than mid-curve readings.
• A pilot run with a few representative samples at two or three dilutions shows which dilution suits the whole study.

How to interpret here: Choose a sample dilution so that expected values fall well inside 1.56 - 100 ng/mL, ideally near the middle of the curve, then multiply by the dilution factor.

Tip: Do not extrapolate beyond the standard curve; run the sample again at another dilution instead.

What it means: Sensitivity is the lowest concentration the assay can distinguish from zero. For this kit the sensitivity is 0.25 ng/mL. Readings close to the detection limit carry more uncertainty than mid-curve readings.

Why it matters:

• It shows whether low levels of the analyte can be detected at all.
• Diluting a sample raises the effective lower limit in the original sample.
• Sensitivity describes the detection limit only; the lowest standard of the curve sets the lowest value that can be quantified reliably.

How to interpret here: Treat readings close to 0.25 ng/mL as near the detection limit; for quantitation, rely on values that fall within the standard curve.

Tip: Use the smallest dilution that keeps samples inside the curve when low levels are expected.