| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Tissue | |
| Disease | Normal tissue |
| Growth properties | |
| Biosafety level | |
| Product format | Frozen |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Human CD56+ Natural Killer (NK) Cells are human frozen primary cells from blood used for innate immune cytotoxicity, activation, and cytokine-response studies. These cells display suspension, round growth characteristics.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Blood
- Growth properties: Suspension, round
- Format: Frozen
- Reported expression/markers: CD56+
- Seeding guidance: 20,000 - 50,000 cells/cm²
- Biosafety level: II
Natural killer cells provide innate immune surveillance and are widely used to study activation-state changes, cytotoxic response programs, and cytokine-driven functional modulation.
Research relevance and current trends
- Primary NK-cell studies commonly evaluate activation, phenotype, and target-cell response under defined cues.
- Investigators examine donor variability and subset composition in functional immune assays.
- Co-culture systems are used to probe reciprocal signaling with tumor, stromal, or antigen-presenting cells.
Common research applications
- Assess activation markers, cytokine responses, or cytotoxicity-associated readouts.
- Use in co-culture systems that require primary innate immune effector cells.
- Profile donor-dependent functional changes following perturbation.
Product-specific data supplied for this listing
- Growth Conditions: PriGrow X Series Medium (TM4124) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. To differentiate Human CD56+ Natural Killer (NK) Cells, supplement TM4124 with 50 ng/ml IL-4 (Z100545) and 50 ng/ml GM-CSF (Z100275) for 4 days. Note: Human CD56+ Natural Killer (NK) Cells do not proliferate, in vitro.
- Seeding Density (cells/cm²): 20,000 - 50,000
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Marker panels should be interpreted together with morphology and functional readouts rather than as a standalone identity measure.
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed (room temperature) growth media. Centrifuge cells at 400xg for 10 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask. Supplement with cytokine, as required.
- Incubate the cells at the recommended conditions.for 4 days.
- Re-feed cells or utilize for experimental purposes as desired.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
