| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carcinoembryonic antigen-related cell adhesion molecule 5;Carcinoembryonic antigen;CEA;Meconium antigen 100;CD66e;CEACAM5;CEA; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: K35-A685 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This one-step sandwich ELISA quantifies human cell adhesion molecule CEACAM5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). The strips are pre-coated with a capture antibody; sample and an HRP-linked detection antibody are incubated together, so the assay needs fewer wash and incubation steps than a standard sandwich ELISA (the supplier markets the Quick format as a 90-minute assay). TMB develops color in proportion to the bound target, and the plate is read at 450 nm.
CEACAM5, better known as carcinoembryonic antigen (CEA, CD66e), is a GPI-anchored glycoprotein of the immunoglobulin superfamily. It mediates cell adhesion with itself and related CEACAMs, and because it is often highly expressed in colorectal and other carcinomas, it is one of the most widely studied tumor-associated antigens.
Kit Components
| 96-well plate precoated with anti-Human CEACAM5 antibody | 1 |
|---|---|
| lyophilized recombinant Human CEACAM5 standard | 20 ng/tube |
| Biotinylated anti-Human CEACAM5 detection antibody (50x) | 100 µL |
| Sample diluent | 30 mL |
| Antibody diluent | 12 mL |
| Wash Buffer (25x) | 20 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Plate Sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 312 pg/mL–20,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human CEACAM5 (Lys35–Ala685), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.0–7.5% (3 samples, n = 16 each) |
| Inter-assay CV | 4.0–8.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.011; 312 = 0.056; 625 = 0.111; 1250 = 0.240; 2500 = 0.381; 5000 = 0.713; 10000 = 1.296; 20000 = 1.967. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.011 | 0.056 | 0.111 | 0.240 | 0.381 | 0.713 | 1.296 | 1.967 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 776 | 1668 | 10176 | 724 | 1662 | 10501 |
| Standard deviation | 39.57 | 124 | 407.04 | 39.09 | 137.94 | 420.04 |
| CV (%) | 4% | 7.5% | 4% | 5.4% | 8.3% | 4% |
Kit components
Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
