| Field | Specification |
|---|---|
| Target | |
| Alternative names | Liver carboxylesterase 1; cholesterol acyltransferase; ACAT; Brain carboxylesterase hBr1; Carboxylesterase 1; CE-1; hCE-1; Cocaine carboxylesterase; Egasyn; HMSE; Methylumbelliferyl-acetate deacetylase 1; Monocyte/macrophage serine esterase; Retinyl ester |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: G18-L567 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human liver carboxylesterase 1 (gene CES1) in cell culture supernatants, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Carboxylesterase 1 (CES1) is a serine hydrolase abundant in the liver that breaks ester bonds in drugs, environmental chemicals and lipids. It activates many ester prodrugs and inactivates others, and it also processes cholesteryl esters and triglycerides, so it is a key enzyme in drug metabolism and lipid research.
Kit Components
| Anti-Human CES1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human CES1 Standard | 2 vials, 50 ng/tube |
| Human CES1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA) |
| Assay range | 0.78 ng/mL–50 ng/mL |
| Sensitivity | <0.1 ng/mL |
| Standard | Recombinant human CES1 (Gly18–Leu567), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.2–5.3% (3 samples, n = 16 each) |
| Inter-assay CV | 5.1–7.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:10. In the supplier’s tests, serum and plasma samples measured about 90 ng/mL.
Example standard curve (OD450, ng/mL): 0 = 0.044; 0.78 = 0.1; 1.56 = 0.164; 3.12 = 0.327; 6.25 = 0.557; 12.5 = 0.959; 25 = 1.776; 50 = 2.49. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.044 | 0.1 | 0.164 | 0.327 | 0.557 | 0.959 | 1.776 | 2.49 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1486 | 5674 | 24323 | 1499 | 6001 | 24678 |
| Standard deviation | 62.41 | 249.66 | 1289.12 | 97.44 | 306.05 | 1801.49 |
| CV (%) | 4.2% | 4.4% | 5.3% | 6.5% | 5.1% | 7.3% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Sun Min Jung et al. (2026) Evaluating Plasma Carboxylesterase 1 as a Protein Biomarker for Pharmacokinetics and Metabolic Dysfunction-Associated Steatotic Liver Disease. Drug Metabolism and Disposition. 10.1016/j.dmd.2026.100307
Wang Huanyu et al. (2024) Increased serum carboxylesterase-1 levels are associated with metabolic dysfunction associated steatotic liver disease and metabolic syndrome in children with obesity. Italian Journal of Pediatrics. 10.1186/s13052-024-01733-7
