| Field | Specification |
|---|---|
| UniProt # | |
| Applications | |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Assay time | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
This ELISA kit applies to the in vitro quantitative determination of Human COL1α1 concentrations in serum, plasma and other biological fluids.
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL1α1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL1α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL1α1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL1α1. You can calculate the concentration of Human COL1α1 in the samples by comparing the OD of the samples to the standard curve.
Performance Specifications
| Sensitivity | 0.19 ng/mL |
|---|---|
| Detection Range | 0.31-20 ng/mL |
| Total Assay Time | 3 h 30 min |
| Compatible Sample Types | Serum, plasma and other biological fluids |
| Species Reactivity | Human |
| Detection Method | Sandwich |
| Precision (CV) | Both intra-CV and inter-CV are < 10%. |
| Recovery Rate | 80%-120% |
| Storage | 2-8℃,12 months |
✓ Research-Grade Validation
Specificity
This kit recognizes Human COL1α1 in samples. No significant cross-reactivity or interference between Human COL1α1 and analogues was observed
Safety & Regulatory
Handle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.
This kit is validated for use with Serum, plasma and other biological fluids. For unlisted matrices (e.g., tissue lysate, urine), perform a spike-and-recovery experiment to confirm assay performance before generating reportable data. Sample dilution in the kit's provided diluent is recommended to minimize matrix interference.
The minimum detectable concentration (sensitivity) of this kit is 0.19 ng/mL. Values below this threshold should be reported as below the limit of detection (<LOD) and should not be extrapolated from the standard curve.
The total assay time from sample addition to absorbance reading is approximately 3 h 30 min, including all incubation, wash, and substrate steps. Hands-on time is typically 1–2 hours; most steps involve passive plate incubation. Plan the assay as a single uninterrupted session for best results.
Standard components of this Sandwich ELISA Kit typically include: pre-coated microplate (96-well strip format), lyophilized or liquid recombinant COL1α1 standard, detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample/standard diluent. Refer to the kit insert or datasheet for the exact component list and storage requirements.
This kit uses colorimetric (TMB/HRP) detection and requires a standard microplate absorbance reader capable of measuring at 450 nm. A reference wavelength of 570 nm or 630 nm is recommended to reduce background. No specialized fluorescence or luminescence reader is needed. Ensure the instrument is calibrated and the plate is clean and free of condensation before reading.
Collagen (COL1) consists of three peptide chains, two of which are α(Ⅰ) chain, COL1α1, and one α(Ⅱ) chain, col1α2. The homology between the two was 60.396%, and there was no obvious cross-reaction.
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
What is the range of enzyme activity of your IL-2 freeze-dried powder
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Double-Layer Nanofibrous Sponge Tube via Electrospun Fiber and Yarn for Promoting Urethral Regeneration (2023) Advanced Fiber Materials. 10.1007/s42765-022-00252-6
Low-dose of Polystyrene microplastics induce cardiotoxicity in mice and human-originated cardiac organoids (2023) ENVIRONMENT INTERNATIONAL. 10.1016/j.envint.2023.108171
Microcrystalline cellulose and boric acid functionalized hydrogel for differentiation of bone marrow stem cells: biocompatibility, hemocompatibility and protein adsorption (2025) INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. 10.1016/j.ijbiomac.2025.146435
Identification of key anti-pulmonary fibrosis components in Hyssopus cuspidatus Boriss. and its mechanism exploration (2025) PHYTOMEDICINE. 10.1016/j.phymed.2025.157676
LARP6 Regulates Keloid Fibroblast Proliferation, Invasion, and Ability to Synthesize Collagen (2022) JOURNAL OF INVESTIGATIVE DERMATOLOGY. 10.1016/j.jid.2022.01.028
Royal Jelly Extracellular Vesicles Enhance Diabetic Wound Healing via Inflammation Modulation, Fibroblast Migration, and Angiogenesis (2025) International Journal of Nanomedicine. 10.2147/IJN.S541042
Resveratrol-Elicited PKC Inhibition Counteracts NOX-Mediated Endothelial to Mesenchymal Transition in Human Retinal Endothelial Cells Exposed to High Glucose (2021) Antioxidants. 10.3390/antiox10020224
An optical pH-sensor integrated microfluidic platform multilayered with bacterial cellulose and gelatin methacrylate to mimic drug-induced lung injury (2023) JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY. 10.1016/j.jiec.2023.01.023
Protective effects of an almond-derived oligosaccharide against UVB-induced photoaging in HaCaT keratinocytes and Zebrafish (2026) Food Bioscience. 10.1016/j.fbio.2026.108834
A study on the anti-senescent effects of flavones derived from Prinsepia utilis Royle seed residue (2024) JOURNAL OF ETHNOPHARMACOLOGY. 10.1016/j.jep.2024.118021
Pirfenidone suppressed triple-negative breast cancer metastasis by inhibiting the activity of the TGF-β/SMAD pathway (2023) JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 10.1111/jcmm.17673
Development of a microneedle patch for delivery of chitin-glucan as a bioactive macromolecule and 3-O-ethyl ascorbic acid for skin rejuvenation (2026) JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY. 10.1016/j.jddst.2026.108232
Enhanced cellular and transdermal delivery of the modified chromatin using gH625 cell-penetrating peptide (2025) Nanomedicine-Nanotechnology Biology and Medicine. 10.1016/j.nano.2025.102884
Paeonia suffruticosa Andrews root extract ameliorates photoaging via regulating IRS1/PI3K/FOXO pathway (2025) Frontiers in Pharmacology. 10.3389/fphar.2025.1520392
A drug-responsive multicellular human spheroid model to recapitulate drug-induced pulmonary fibrosis (2022) Biomedical Materials. 10.1088/1748-605X/ac73cd
Protective effects of avocado peel and seed extracts against UVB-damaged fibroblasts for the development of an anti-photoaging nanoemulgel (2025) Scientific Reports. 10.1038/s41598-025-13679-9
Enhanced cardioprotective efficacy of Rosmarinus officinalis-loaded polydopamine nanoparticles for targeted therapy of myocardial infarction (2025) JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION. 10.1080/09205063.2025.2590717
From Baker’s Yeast to Skin Rejuvenation: Insights into the Anti-Wrinkle Properties of Chitin–Glucans Extracted from Saccharomyces cerevisiae (2026) ChemEngineering. 10.3390/chemengineering10030033
PLOD1 promotes gastric cancer metastasis and immune escape by mediating extracellular matrix remodeling (2025) Discover Oncology. 10.1007/s12672-025-03774-8
LncRNA SNHG14 Participates in the Development of Chronic Obstructive Pulmonary Disease by Targeting the miR-150-5p/BASP1 Axis (2026) JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY. 10.1002/jbt.70843
Protective Effects of Annatto Tocotrienol and Palm Tocotrienol-Rich Fraction on Chondrocytes Exposed to Monosodium Iodoacetate (2021) Applied Sciences-Basel. 10.3390/app11209643
Apigenin inhibits epithelial mesenchymal transition in renal tubular epithelial cells through PI3K/AKT and NF-κB pathways for treating renal fibrosis (2024) GENE. 10.1016/j.gene.2024.149056
Lipopolysaccharide induces epithelial-mesenchymal transition of alveolar epithelial cells cocultured with macrophages possibly via the JAK2/STAT3 signaling pathway. (2019) HUMAN & EXPERIMENTAL TOXICOLOGY. 10.1177/0960327119881678
In-vitro study on type I collagen synthesis in low-level laser therapy on the early ligament fibroblasts’ healing process (2024) LASERS IN MEDICAL SCIENCE. 10.1007/s10103-024-04151-7
Dendrobium officinale Extract Fermented with Lactobacillus plantarum GT-17F Enhances the Protection of UV-Mediated Photoaging (2023) BIOLOGICAL & PHARMACEUTICAL BULLETIN. 10.1248/bpb.b23-00373
