| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cartilage oligomeric matrix protein;COMP;Thrombospondin-5;TSP5;COMP; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: Q21-A757 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human cartilage oligomeric matrix protein (gene COMP) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Cartilage oligomeric matrix protein (COMP, thrombospondin-5) is a pentameric extracellular matrix glycoprotein abundant in cartilage, tendon and ligament. It binds collagens and fibronectin to stabilize the matrix, mutations cause pseudoachondroplasia, and COMP is often measured as a marker of cartilage turnover in arthritis research.
Kit Components
| Anti-Human COMP Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human COMP Standard | 2 vials, 10 ng/tube |
| Human COMP Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human COMP (Gln21–Ala757), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.2–6.9% (3 samples, n = 16 each) |
| Inter-assay CV | 4.9–7.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:5000. In the supplier’s tests, serum samples measured about 4000 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.02; 156 = 0.066; 312 = 0.12; 625 = 0.231; 1250 = 0.462; 2500 = 0.918; 5000 = 1.816; 10000 = 2.572. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.02 | 0.066 | 0.12 | 0.231 | 0.462 | 0.918 | 1.816 | 2.572 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 233 | 1997 | 4935 | 255 | 1893 | 5413 |
| Standard deviation | 9.78 | 137.79 | 246.75 | 12.49 | 138.18 | 281.47 |
| CV (%) | 4.2% | 6.9% | 5% | 4.9% | 7.3% | 5.2% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Matthew S. Harkey et al. (2020) Sex-Specific Associations between Cartilage Structure and Metabolism at Rest and Acutely Following Walking and Drop-Landing. Cartilage. 10.1177/1947603520959386
Akinmade Akinola et al. (2021) Serum cartilage oligomeric matrix protein as a biomarker for predicting development and progression of knee osteoarthritis. International Orthopaedics. 10.1007/s00264-021-04943-4
Domen Plut et al. (2020) Detection and evaluation of haemophilic arthropathy: Which tools may be considered more reliable. Haemophilia. 10.1111/hae.14153
Jian Wu et al. (2017) Platelet-rich plasma inhibits Wnt/β-catenin signaling in rabbit cartilage cells activated by IL-1β. International Immunopharmacology. 10.1016/j.intimp.2017.12.031
Pu Wang et al. (2015) Effects of Whole Body Vibration Exercise associated with Quadriceps Resistance Exercise on functioning and quality of life in patients with knee osteoarthritis: a randomized controlled trial. Clinical Rehabilitation. 10.1177/0269215515607970
Brittney A. Luc-Harkey et al. (2018) Lesser lower extremity mechanical loading associates with a greater increase in serum cartilage oligomeric matrix protein following walking in individuals with anterior cruciate ligament reconstruction. Clinical Biomechanics. 10.1016/j.clinbiomech.2018.09.024
