| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Expression System | |
| Gene ID | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: S672-R748 |
| Product Type | |
| Reactivity | |
| Sample Type(s) | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine |
| Sensitivity | |
| Storage | |
| Target | |
| UniProt # |
Background
Human complement C3a (C3) is widely studied as a molecular readout in experimental models where changes in protein abundance reflect underlying biology. This target is frequently investigated in Molecular & Cellular Biology research contexts. This analyte is often discussed in the context of plasma protein and inflammation-linked pathways. Inflammation and coagulation networks include abundant plasma proteins, regulators, and cleavage products that can change with immune activation and tissue damage.
Biological context
In experimental systems, protein abundance can reflect regulated expression, secretion, processing, or clearance. Interpreting changes benefits from considering compartment (cell-associated vs soluble), the time scale of regulation, and whether complexes or modified forms contribute to the measured signal.
Why it matters in research
- Systems-level readout: Quantification supports comparisons across conditions, time points, and treatment groups.
- Mechanistic interpretation: Pairing with upstream regulators and downstream markers helps contextualize changes.
- Biomarker-style profiling: Measuring panels of related analytes can improve interpretability in complex models.
Sample data
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.005 | 0.041 | 0.084 | 0.178 | 0.355 | 0.704 | 1.109 | 2.147 |
Kit components
| Catalog number | Description | Quantity | Storage of opened/reconstituted material |
|---|---|---|---|
| EZ2145-CA | Mouse anti-human complement C3a monoclonal antibody (Capture Antibody) | 500 µl, 4 µg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
| EZ2145-DA | Biotinylated goat anti-human complement C3a polyclonal antibody (Detection Antibody) | 500 µl (recommended dilution 1:100) | |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
| EZ2145-ST | Lyophilized recombinant human complement C3a standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
Materials required but not provided
- Microplate reader in standard size.
- Automated plate washer.
- Incubator.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- 96 well microplate (Cat# AR1100)
- Plate Sealers.
- Capture Antibody Diluent: PBS.
- Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- Stop Solution: 2 N H2SO4 (Cat# AR1105)
- Wash Buffer (PBS and PBS-T). Recipes:
- PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
- PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
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