| Field | Specification |
|---|---|
| Target | |
| Alternative names | Growth-regulated alpha protein;C-X-C motif chemokine 1;GRO-alpha (1-73);Melanoma growth stimulatory activity;MGSA;Neutrophil-activating protein 3;NAP-3;GRO-alpha (4-73);GRO-alpha (5-73);GRO-alpha (6-73);CXCL1;GRO, GRO1, GROA, MGSA, SCYB1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: A35-N107 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human growth-regulated alpha protein (gene CXCL1) in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
CXCL1 (GRO-α, KC in mouse) is a CXC chemokine that attracts and activates neutrophils through the CXCR2 receptor. It is produced by macrophages, endothelial cells and epithelial cells during inflammation and infection, and it is widely measured in studies of acute inflammation, tissue injury and tumor-associated neutrophil recruitment.
Kit Components
| Anti-Human CXCL1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human CXCL1 Standard | 2 vials, 10 ng/tube |
| Human CXCL1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
| Assay range | 15.6 pg/mL–1,000 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant human CXCL1 (Ala35–Asn107), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.0–7.2% (3 samples, n = 16 each) |
| Inter-assay CV | 5.3–8.2% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured about 200 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.024; 15.6 = 0.079; 31.2 = 0.136; 62.5 = 0.229; 125 = 0.445; 250 = 0.809; 500 = 1.538; 1000 = 2.545. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.024 | 0.079 | 0.136 | 0.229 | 0.445 | 0.809 | 1.538 | 2.545 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 34 | 194 | 451 | 36 | 204 | 438 |
| Standard deviation | 2.44 | 9.7 | 29.31 | 2.95 | 10.81 | 36.51 |
| CV (%) | 7.2% | 5% | 6.5% | 8.2% | 5.3% | 8.2% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Wee Zhen Ning et al. (2015) IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel. Nature Communications. 10.1038/ncomms9746
Yang Su et al. (2019) Interleukin-17 receptor D constitutes an alternative receptor for interleukin-17A important in psoriasis-like skin inflammation. Science Immunology. 10.1126/sciimmunol.aau9657
Hokeung Tang et al. (2022) HSP90/IKK-rich small extracellular vesicles activate pro-angiogenic melanoma-associated fibroblasts via the NF-κB/CXCL1 axis. Cancer Science. 10.1111/cas.15271
Xu Ying et al. (2023) Single-cell transcriptomes reveal a molecular link between diabetic kidney and retinal lesions. Communications Biology. 10.1038/s42003-023-05300-4
Le Naour Augustin et al. (2019) Tumor cells educate mesenchymal stromal cells to release chemoprotective and immunomodulatory factors. Journal of Molecular Cell Biology. 10.1093/jmcb/mjz090
Chen Yuwen et al. (2022) Multiplex Assessment of Serum Chemokines CCL2, CCL5, CXCL1, CXCL10, and CXCL13 Following Traumatic Brain Injury. Inflammation. 10.1007/s10753-022-01729-7
Man Xiaxia et al. (2022) High expression level of CXCL1/GROα is linked to advanced stage and worse survival in uterine cervical cancer and facilitates tumor cell malignant processes. BMC Cancer. 10.1186/s12885-022-09749-0
Kong Jian et al. (2012) After insufficient radiofrequency ablation, tumor-associated endothelial cells exhibit enhanced angiogenesis and promote invasiveness of residual hepatocellular carcinoma. Journal of Translational Medicine. 10.1186/1479-5876-10-230
Yunlong Shan et al. (2019) Preclinical development of GR1501, a human monoclonal antibody that neutralizes interleukin-17A. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2019.07.078
Shiliang Gui et al. (2025) Enhanced expression of CXCL1 in renal cell carcinoma facilitates tumor cell malignancy via PI3K/AKT‑dependent mechanisms. Oncology Letters. 10.3892/ol.2025.15372
