| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein;Gamma-IP10;IP-10;Small-inducible cytokine B10;CXCL10 (1-73);CXCL10;INP10, SCYB10; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: V22-P98 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Saliva, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human C-X-C motif chemokine 10 (gene CXCL10) in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
CXCL10 (IP-10) is an interferon-γ-inducible chemokine that binds the receptor CXCR3. It attracts activated T cells, NK cells and monocytes to sites of infection and inflammation and also has angiostatic effects. CXCL10 is commonly measured as a marker of interferon-driven immune responses in viral infection, autoimmunity and transplant research.
Kit Components
| Anti-Human CXCL10 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human CXCL10 Standard | 2 vials, 10 ng/tube |
| Human CXCL10 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant human CXCL10 (Val22–Pro98), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.2–7.9% (3 samples, n = 16 each) |
| Inter-assay CV | 5.5–8.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured 80–133 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.038; 31.2 = 0.136; 62.5 = 0.206; 125 = 0.345; 250 = 0.535; 500 = 0.998; 1000 = 1.625; 2000 = 2.684. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.038 | 0.136 | 0.206 | 0.345 | 0.535 | 0.998 | 1.625 | 2.684 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 60 | 243 | 879 | 64 | 223 | 865 |
| Standard deviation | 3.24 | 19.19 | 45.7 | 4.28 | 19.4 | 47.57 |
| CV (%) | 5.4% | 7.9% | 5.2% | 6.7% | 8.7% | 5.5% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Min He et al. (2024) Construction of self-driving anti-αFR CAR-engineered NK cells based on IFN-γ and TNF-α synergistically induced high expression of CXCL10. Neoplasia. 10.1016/j.neo.2024.101065
Yawen Zheng et al. (2025) Impact of MEK inhibition on T-cell infiltration and function after radiotherapy in KRAS-mutant lung cancer. Frontiers in Immunology. 10.3389/fimmu.2025.1663502
Stefano Quarta et al. (2026) Omega-3 PUFAs Reduce Inflammation by Targeting NRF2 and NF-κB activity in an Ex-Vivo Model of Cardiac mature Adipocytes and adipose derived stem cells from Atherosclerotic Patients. Food & Function. 10.1039/D5FO03219A
Chen Yuwen et al. (2022) Multiplex Assessment of Serum Chemokines CCL2, CCL5, CXCL1, CXCL10, and CXCL13 Following Traumatic Brain Injury. Inflammation. 10.1007/s10753-022-01729-7
Yan Lv et al. (2024) Differential alterations of CXCR3, CXCR5 and CX3CR1 in patients with immune thrombocytopenia. Cytokine. 10.1016/j.cyto.2024.156684
Stefania Farcomeni et al. (2021) Short- and Long-Term Immunological Responses in Chronic HCV/HIV Co-Infected Compared to HCV Mono-Infected Patients after DAA Therapy. Pathogens. 10.3390/pathogens10111488
Honggang Wang et al. (2019) Blocking CXCR3 with AMG487 ameliorates the blood-retinal barrier disruption in diabetic mice through anti-oxidative. Life Sciences. 10.1016/j.lfs.2019.04.016
Hoffmann Jessica Alana et al. (2023) Prediction of spontaneous preterm birth using CCL2 and CXCL10 in maternal serum of symptomatic high-risk pregnant women: a prospective cohort study. BMC Pregnancy and Childbirth. 10.1186/s12884-023-06016-3
Xiang Cai et al. (2024) JMJD2A promotes the development of castration-resistant prostate cancer by activating androgen receptor enhancer and inhibiting the cGAS-STING pathway. Molecular Carcinogenesis. 10.1002/mc.23753
Hadi Peeridogaheh et al. (2019) The impact of Aggregatibacter actinomycetemcomitans biofilm-derived effectors following antimicrobial photodynamic therapy on cytokine production in human gingival fibroblasts. Photodiagnosis and Photodynamic Therapy. 10.1016/j.pdpdt.2019.05.025
