| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C motif chemokine 13;Angie;B cell-attracting chemokine 1;BCA-1;B lymphocyte chemoattractant;CXC chemokine BLC;Small-inducible cytokine B13;CXCL13;BCA1, BLC, SCYB13; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: V23-R94 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma, Saliva |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human C-X-C motif chemokine 13 (gene CXCL13) in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
CXCL13 (BLC) is a chemokine that attracts B cells through the receptor CXCR5. Produced by stromal cells in lymphoid follicles, it organizes B-cell follicles and guides follicular helper T cells. CXCL13 is also studied in ectopic lymphoid structures that form in chronic inflammation, autoimmunity and some tumors.
Kit Components
| Anti-Human CXCL13 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human CXCL13 Standard | 2 vials, 10 ng/tube |
| Human CXCL13 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva |
| Assay range | 15.6 pg/mL–1,000 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant human CXCL13 (Val23–Arg94), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 5.6–7.8% (3 samples, n = 16 each) |
| Inter-assay CV | 6.8–8.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured 31–302 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.025; 15.6 = 0.097; 31.2 = 0.166; 62.5 = 0.292; 125 = 0.491; 250 = 0.934; 500 = 1.51; 1000 = 2.126. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.025 | 0.097 | 0.166 | 0.292 | 0.491 | 0.934 | 1.51 | 2.126 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 32 | 216 | 441 | 30 | 232 | 416 |
| Standard deviation | 2.2 | 16.84 | 24.69 | 2.19 | 20.64 | 27.3%8 |
| CV (%) | 6.9% | 7.8% | 5.6% | 7.3% | 8.9% | 6.8% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Zhi Wang et al. (2026) Myelin injury after ischemic stroke mediated by PLA2G4A-driven lipid imbalance via impairment of microglial phagocytosis. Genes & Diseases. 10.1016/j.gendis.2026.102263
Molina-Alejandre Marta et al. (2026) Plasmatic CXCL13 as a biomarker of primary resistance to immunotherapy and impaired peripheral immunity in non-small cell lung cancer. Biomarker Research. 10.1186/s40364-026-00929-9
Zhicheng Liu et al. (2026) Triamcinolone acetonide nanoparticles ameliorate interstitial cystitis and anxiety/depression by targeting the bladder–metabolic–brain axis. Materials Today Bio. 10.1016/j.mtbio.2026.103112
Chuan Wang et al. (2023) Circulating Tfr/Tfh cell imbalance may contribute to the immunopathogenesis of pemphigus. Journal of the European Academy of Dermatology and Venereology. 10.1111/jdv.19338
Chuan Wang et al. (2025) Insufficient CXCL13 secretion in leprosy foamy macrophages attenuates lymphocyte recruitment and antimicrobial protein production. Frontiers in Immunology. 10.3389/fimmu.2025.1541954
Chen Yuwen et al. (2022) Multiplex Assessment of Serum Chemokines CCL2, CCL5, CXCL1, CXCL10, and CXCL13 Following Traumatic Brain Injury. Inflammation. 10.1007/s10753-022-01729-7
Yan Lv et al. (2024) Differential alterations of CXCR3, CXCR5 and CX3CR1 in patients with immune thrombocytopenia. Cytokine. 10.1016/j.cyto.2024.156684
Dongdong Li et al. (2021) Diagnostic role of CXCL13 and CSF serology in patients with neurosyphilis. Sexually Transmitted Infections. 10.1136/sextrans-2020-054778
