Human DNA Polymerase Beta Assay Kit

SKU:BHT16300003
Overview
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Fluorescence assay kit for human DNA polymerase beta, a base excision repair enzyme and anticancer target. Measure polymerase activity or test inhibitors in 96-well plates. Choose the Kit without enzyme or the Kit Plus with human DNA polymerase beta, 100 assays each.
Assay Target Human DNA polymerase beta activity
Detection Method Fluorescence
Plate Format 96-well plate
Options selector
Catalog no. Format
DPB100K Kit (enzyme not included)
DPB100KE Kit Plus (enzyme included)
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Format: Kit (enzyme not included), Kit Plus (enzyme included)
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Please contact us to confirm storage conditions.
  • Shipping: Shipped frozen.
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Organism Human
Applications
  • Enzyme Activity
  • Compound Profiling
Assay type
  • Enzyme Activity Assay
  • Inhibitor Screening Assay
Assay target Human DNA polymerase beta activity
Assay area Anticancer Drug Discovery
Target type DNA Polymerase
Detection method
  • Fluorescence
Instrument required Fluorescence microplate reader
Plate format 96-well plate
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.
Catalog no. (Mfr.) DPB100K | DPB100KE
Main SKU BHT16300003
Drug Target Assay Kit

Human DNA polymerase beta, an anticancer drug target, works in base excision repair (BER): using the opposite strand as a template, it fills short single-strand gaps of 1 to 6 bases. This kit measures its polymerase activity with a DNA template and dNTPs.

Kit vs Kit Plus

DPB100KE includes the human DNA polymerase beta enzyme; DPB100K has the same reagents without the enzyme. Both are for 100 assays.

Kit (enzyme not included) (DPB100K) 100 assays
Kit Plus (enzyme included) (DPB100KE) 100 assays

Kit Components

Component Kit (enzyme not included) (DPB100K) Kit Plus (enzyme included) (DPB100KE)
10 x Buffer BP Included 800 µl
100 x DNA template Included 55 µl
100 x dNTP mix Included 55 µl
Reagent U Included 22 ml
10 x fluorescence dye Included 420 µl
100 x human DNA polymerase beta Not included 55 µl

Component volumes are stated for DPB100KE; DPB100K contains all the assay reagents except the enzyme.

Assay Principle

The assay detects the DNA made by human DNA polymerase beta from a DNA template and dNTPs; a fluorescence dye is used for the readout.

Specifications

Assay Target Human DNA polymerase beta activity
Target Type DNA Polymerase
Organism Human
Assay Type Enzyme Activity Assay; Inhibitor Screening Assay
Detection Method Fluorescence
Plate Format 96-well plate
Instrument Required Fluorescence microplate reader
Size 100 assays
Storage Please contact us to confirm storage conditions.
Shipping Shipped frozen.

Protocol

The manufacturer's protocol describes the procedure and reaction volumes.

  • Human DNA polymerase beta assay (PDF, see Documents): Covers catalog # DPB100K, DPB100KE.

Safety & Handling

For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The Human DNA Polymerase Beta Assay Kit is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.
Q.What does this kit measure?
A.It measures the activity of human DNA polymerase beta by detecting the DNA it synthesizes from a DNA template and dNTPs with a fluorescence dye. It can also be used to test DNA polymerase beta inhibitors.
Q.What is the difference between the options?
A.DPB100KE includes the human DNA polymerase beta enzyme; DPB100K has the same reagents without the enzyme. Both are for 100 assays.
Q.Is a protocol available?
A.Yes. The manufacturer's protocol (Human DNA polymerase beta assay, linked under Documents) covers catalog # DPB100K, DPB100KE.
Q.How should the kit be stored?
A.The Human DNA Polymerase Beta Assay Kit is shipped frozen. Please contact us for long-term storage conditions.

Can’t find the exact format you need? We can help you source or customize this human DNA polymerase beta activity assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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