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Human DNA polymerase beta, an anticancer drug target, works in base excision repair (BER): using the opposite strand as a template, it fills short single-strand gaps of 1 to 6 bases. This kit measures its polymerase activity with a DNA template and dNTPs.
Kit vs Kit Plus
DPB100KE includes the human DNA polymerase beta enzyme; DPB100K has the same reagents without the enzyme. Both are for 100 assays.
| Kit (enzyme not included) (DPB100K) | 100 assays |
|---|---|
| Kit Plus (enzyme included) (DPB100KE) | 100 assays |
Kit Components
| Component | Kit (enzyme not included) (DPB100K) | Kit Plus (enzyme included) (DPB100KE) |
|---|---|---|
| 10 x Buffer BP | Included | 800 µl |
| 100 x DNA template | Included | 55 µl |
| 100 x dNTP mix | Included | 55 µl |
| Reagent U | Included | 22 ml |
| 10 x fluorescence dye | Included | 420 µl |
| 100 x human DNA polymerase beta | Not included | 55 µl |
Component volumes are stated for DPB100KE; DPB100K contains all the assay reagents except the enzyme.
Assay Principle
The assay detects the DNA made by human DNA polymerase beta from a DNA template and dNTPs; a fluorescence dye is used for the readout.
Specifications
| Assay Target | Human DNA polymerase beta activity |
|---|---|
| Target Type | DNA Polymerase |
| Organism | Human |
| Assay Type | Enzyme Activity Assay; Inhibitor Screening Assay |
| Detection Method | Fluorescence |
| Plate Format | 96-well plate |
| Instrument Required | Fluorescence microplate reader |
| Size | 100 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
The manufacturer's protocol describes the procedure and reaction volumes.
- Human DNA polymerase beta assay (PDF, see Documents): Covers catalog # DPB100K, DPB100KE.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this human DNA polymerase beta activity assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.