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| Assay type | |
| Assay target | |
| Assay area | |
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Drug Target Assay Kit
Human DNA polymerase theta is an anticancer drug target. This kit measures its helicase (DNA unwinding) activity with a DNA duplex substrate and ATP.
Kit Components
| Component | DU050PQ |
|---|---|
| 10 x Assay buffer | 300 µl |
| 100 x DNA duplex | 25 µl |
| 100 x Trapping DNA | 125 µl |
| 100 x human DNA polymerase theta N-terminal domain | 25 µl |
| 100 x ATP | 25 µl |
Specifications
| Assay Target | Human Pol theta helicase unwinding |
|---|---|
| Target Type | Helicase |
| Organism | Human |
| Assay Type | Enzyme Activity Assay |
| Size | 50 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Safety & Handling
For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The Human DNA Polymerase Theta Helicase Assay Kit Plus is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.
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Q.What does this kit measure?
A.It measures the DNA unwinding (helicase) activity of the human DNA polymerase theta N-terminal domain on a DNA duplex substrate in the presence of ATP.
Q.What is included in the kit?
A.The Human DNA Polymerase Theta Helicase Assay Kit Plus (50 assays) includes 10 x assay buffer (300 µl), 100 x DNA duplex (25 µl), 100 x trapping DNA (125 µl), 100 x human DNA polymerase theta N-terminal domain (25 µl) and 100 x ATP (25 µl).
Q.Is a protocol available?
A.A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Q.How should the kit be stored?
A.The Human DNA Polymerase Theta Helicase Assay Kit Plus is shipped frozen. Please contact us for long-term storage conditions.
Can’t find the exact format you need? We can help you source or customize this human Pol theta helicase unwinding assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.
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